Maulidyanti Ellies Tunjung Sari, Purwaningsih Nur Vita, Ainutajriani Ainutajriani, Widyastuti Rahma
Department of Medical Technology Laboratory, Faculty Health Science, University Muhammadiyah of Surabaya, Surabaya, Indonesia.
Malays J Med Sci. 2024 Apr;31(2):153-158. doi: 10.21315/mjms2024.31.2.13. Epub 2024 Apr 23.
The quality of laboratory test results depends on various factors, including sample type selection. Blood samples, such as whole blood, plasma and serum are commonly used for most clinical laboratory examinations. D-dimer parameters are frequently analysed in haematology laboratories and serve as biomarkers for coagulation activation and fibrinolysis. This study aimed to assess the impact of using different sample types on the quality of D-dimer test results.
An observational analytical method was used. D-dimer examination was performed using the fluorescent lateral flow immunoassay method. The study sample consisted of 26 participants aged between 18 years old and 22 years old who had no blood disorders. Whole blood and ethylenediaminetetraacetic acid (EDTA) plasma samples were used for the examination of D-dimer levels.
D-dimer levels in 26 participants using whole blood samples had a mean value of 0.23 mg/L (230 ng/mL), while plasma samples yielded a mean value of 0.14 mg/L (140 ng/mL). D-dimer levels obtained from whole blood samples were higher than plasma samples but remained within the normal range of 0 mg/L-0.5 mg/L (0 ng/mL-500 ng/mL).
The results showed that whole blood samples were more practical than plasma samples. Nevertheless, plasma samples gave results within the normal range of D-dimer values.
实验室检测结果的质量取决于多种因素，包括样本类型的选择。血液样本，如全血、血浆和血清，常用于大多数临床实验室检查。D - 二聚体参数在血液学实验室中经常被分析，并作为凝血激活和纤维蛋白溶解的生物标志物。本研究旨在评估使用不同样本类型对D - 二聚体检测结果质量的影响。
采用观察性分析方法。使用荧光侧向流动免疫分析法进行D - 二聚体检测。研究样本包括26名年龄在18岁至22岁之间且无血液疾病的参与者。使用全血和乙二胺四乙酸（EDTA）血浆样本检测D - 二聚体水平。
26名参与者使用全血样本检测的D - 二聚体水平平均值为0.23 mg/L（230 ng/mL），而血浆样本的平均值为0.14 mg/L（140 ng/mL）。全血样本获得的D - 二聚体水平高于血浆样本，但仍在0 mg/L - 0.5 mg/L（0 ng/mL - 500 ng/mL）的正常范围内。
结果表明全血样本比血浆样本更实用。然而，血浆样本的检测结果在D - 二聚体值的正常范围内。
