Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
ACS Appl Mater Interfaces. 2024 May 15;16(19):25333-25342. doi: 10.1021/acsami.4c02721. Epub 2024 May 2.
Mycotoxin contamination in food and the environment seriously harms human health. Sensitive and timely detection of mycotoxins is crucial. Here, we report a dual-functional hybrid membrane with absorptivity and responsiveness for fluorescent-quantitative detection of mycotoxin aflatoxin B (AFB). A biomineralization-inspired and microwave-accelerated fabrication method was established to prepare a hybrid membrane with a metal-organic framework (MOF) loaded in high density. The MOF presented high efficiency in capturing AFB and showed fluorescence intensity alteration simultaneously, enabling a dual adsorption-response mode. Deriving from the inherent porous structure of the hybrid membrane and the absorptive/responsive ability of the loaded MOF, a filtration-enhanced detection mode was elaborated to provide a 1.67-fold signal increase compared with the conventional soaking method. Therefore, the hybrid membrane exhibited a rapid response time of 10 min and a low detection limit of 0.757 ng mL, superior to most analogues in rapidity and sensitivity. The hybrid membrane also presented superior specificity, reproducibility, and anti-interference ability and even performed well in extreme environments such as strong acid or alkaline, satisfying the practical requirements for facile and in-field detection. Therefore, the membrane had strong applicability in chicken feed samples, with a detection recovery between 70.6% and 101%. The hybrid membrane should have significant prospects in the rapid and in-field inspection of mycotoxins for agriculture and food.
食品和环境中的真菌毒素污染严重危害人类健康。对真菌毒素进行敏感、及时的检测至关重要。在这里，我们报告了一种具有吸收性和响应性的双重功能混合膜，用于荧光定量检测真菌毒素黄曲霉毒素 B（AFB）。建立了一种基于生物矿化的微波加速制备方法，以高密度制备负载金属有机骨架（MOF）的混合膜。该 MOF 对 AFB 具有高效的捕获能力，同时表现出荧光强度的变化，从而实现了双吸附-响应模式。源于混合膜的固有多孔结构和负载 MOF 的吸收/响应能力，阐述了一种过滤增强型检测模式，与传统浸泡法相比，信号增加了 1.67 倍。因此，混合膜具有 10 min 的快速响应时间和 0.757 ng mL 的低检测限，在快速性和灵敏度方面优于大多数类似物。混合膜还表现出优异的特异性、重现性和抗干扰能力，甚至在强酸或强碱等极端环境下也能很好地发挥作用，满足了简便和现场检测的实际要求。因此，该膜在鸡肉饲料样品中的检测具有很强的适用性，检测回收率在 70.6%至 101%之间。该混合膜在农业和食品中真菌毒素的快速现场检测方面具有广阔的应用前景。
