Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Medical Scientist Training Program, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Physiol Rep. 2024 May;12(9):e15997. doi: 10.14814/phy2.15997.
Voluntary or forced exercise training in mice is used to assess functional capacity as well as potential disease-modifying effects of exercise over a range of cardiovascular disease phenotypes. Compared to voluntary wheel running, forced exercise training enables precise control of exercise workload and volume, and results in superior changes in cardiovascular performance. However, the use of a shock grid with treadmill-based training is associated with stress and risk of injury, and declining compliance with longer periods of training time for many mouse strains. With these limitations in mind, we designed a novel, high-intensity interval training modality (HIIT) for mice that is carried out on a rotarod. Abbreviated as RotaHIIT, this protocol establishes interval workload intensities that are not time or resource intensive, maintains excellent training compliance over time, and results in improved exercise capacity independent of sex when measured by treadmill graded exercise testing (GXT) and rotarod specific acceleration and endurance testing. This protocol may therefore be useful and easily implemented for a broad range of research investigations. As RotaHIIT training was not associated cardiac structural or functional changes, or changes in oxidative capacity in cardiac or skeletal muscle tissue, further studies will be needed to define the physiological adaptations and molecular transducers that are driving the training effect of this exercise modality.
在小鼠中进行自愿或强制运动训练，可用于评估功能能力，以及运动对一系列心血管疾病表型的潜在疾病修正作用。与自愿轮跑相比，强制运动训练能够精确控制运动工作量和量，并且更能改善心血管性能的变化。然而，跑步机训练中使用电击网格会导致应激和受伤风险，并降低许多小鼠品系对更长时间训练的依从性。考虑到这些限制，我们为小鼠设计了一种新颖的高强度间歇训练模式（HIIT），该模式在转棒上进行。简称为 RotaHIIT，该方案确定了间隔工作强度，既不耗时也不耗费资源，随着时间的推移保持了出色的训练依从性，并且通过跑步机分级运动测试（GXT）和转棒特定加速度和耐力测试测量，独立于性别改善了运动能力。因此，该方案可能对广泛的研究调查有用且易于实施。由于 RotaHIIT 训练与心脏结构或功能变化或心脏和骨骼肌组织中的氧化能力变化无关，因此需要进一步的研究来确定驱动这种运动模式训练效果的生理适应和分子转导器。
