• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Stm1通过休眠核糖体对真核起始因子5A、真核延伸因子2和转运RNA的保护作用。

Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes.

作者信息

Du Mengtan, Li Xin, Dong Wanlin, Zeng Fuxing

机构信息

Department of Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.

Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, China.

出版信息

Front Mol Biosci. 2024 Apr 18;11:1395220. doi: 10.3389/fmolb.2024.1395220. eCollection 2024.

DOI:10.3389/fmolb.2024.1395220
PMID:38698775
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11063288/
Abstract

Dormant ribosomes are typically associated with preservation factors to protect themselves from degradation under stress conditions. Stm1/SERBP1 is one such protein that anchors the 40S and 60S subunits together. Several proteins and tRNAs bind to this complex as well, yet the molecular mechanisms remain unclear. Here, we reported the cryo-EM structures of five newly identified Stm1/SERBP1-bound ribosomes. These structures highlighted that eIF5A, eEF2, and tRNA might bind to dormant ribosomes under stress to avoid their own degradation, thus facilitating protein synthesis upon the restoration of growth conditions. In addition, Ribo-seq data analysis reflected the upregulation of nutrient, metabolism, and external-stimulus-related pathways in the strain, suggesting possible regulatory roles of Stm1. The knowledge generated from the present work will facilitate in better understanding the molecular mechanism of dormant ribosomes.

摘要

休眠核糖体通常与保护因子相关联，以在应激条件下保护自身免受降解。Stm1/SERBP1就是这样一种将40S和60S亚基锚定在一起的蛋白质。还有几种蛋白质和tRNA也结合到这个复合物上，但其分子机制仍不清楚。在此，我们报道了五个新鉴定的与Stm1/SERBP1结合的核糖体的冷冻电镜结构。这些结构突出表明，eIF5A、eEF2和tRNA可能在应激条件下与休眠核糖体结合，以避免自身降解，从而在生长条件恢复时促进蛋白质合成。此外，核糖体测序数据分析反映了该菌株中营养、代谢和外部刺激相关途径的上调，表明Stm1可能具有调控作用。本研究产生的知识将有助于更好地理解休眠核糖体的分子机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/20b04eba6ff8/fmolb-11-1395220-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/b2b88f47a079/fmolb-11-1395220-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/eecbfd04d26c/fmolb-11-1395220-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/4e68f9b0a2d7/fmolb-11-1395220-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/232223676e30/fmolb-11-1395220-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/20b04eba6ff8/fmolb-11-1395220-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/b2b88f47a079/fmolb-11-1395220-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/eecbfd04d26c/fmolb-11-1395220-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/4e68f9b0a2d7/fmolb-11-1395220-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/232223676e30/fmolb-11-1395220-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fadd/11063288/20b04eba6ff8/fmolb-11-1395220-g005.jpg

相似文献

1
Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes.Stm1通过休眠核糖体对真核起始因子5A、真核延伸因子2和转运RNA的保护作用。
Front Mol Biosci. 2024 Apr 18;11:1395220. doi: 10.3389/fmolb.2024.1395220. eCollection 2024.
2
TORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes.TORC1 磷酸化并抑制核糖体保护因子 Stm1 以激活休眠核糖体。
EMBO J. 2023 Mar 1;42(5):e112344. doi: 10.15252/embj.2022112344. Epub 2023 Jan 24.
3
Tight interaction of eEF2 in the presence of Stm1 on ribosome.eEF2 在核糖体上与 Stm1 的紧密相互作用。
J Biochem. 2018 Mar 1;163(3):177-185. doi: 10.1093/jb/mvx070.
4
Evidence for a Negative Cooperativity between eIF5A and eEF2 on Binding to the Ribosome.eIF5A与eEF2结合核糖体时存在负协同效应的证据。
PLoS One. 2016 Apr 26;11(4):e0154205. doi: 10.1371/journal.pone.0154205. eCollection 2016.
5
Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.酵母 Lso2 和与人 CCDC124 结合的休眠核糖体的结构与功能。
PLoS Biol. 2020 Jul 20;18(7):e3000780. doi: 10.1371/journal.pbio.3000780. eCollection 2020 Jul.
6
High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation.热嗜酸性真核 80S 核糖体的高分辨率结构揭示了转位的原子细节。
Nat Commun. 2022 Jan 25;13(1):476. doi: 10.1038/s41467-022-27967-9.
7
Studies on native ribosomal subunits from rat liver. Evidence for activities associated with native 40S subunits that affect the interaction with acetylphenylalanyl-tRNA, methionyl-tRNAf, and 60S subunits.对大鼠肝脏天然核糖体亚基的研究。与天然40S亚基相关的活性影响其与乙酰苯丙氨酰 - tRNA、甲硫氨酰 - tRNAf及60S亚基相互作用的证据。
Biochemistry. 1975 Dec 2;14(24):5328-35. doi: 10.1021/bi00695a017.
8
A molecular network of conserved factors keeps ribosomes dormant in the egg.一个保守因子的分子网络使卵中的核糖体处于休眠状态。
Nature. 2023 Jan;613(7945):712-720. doi: 10.1038/s41586-022-05623-y. Epub 2023 Jan 18.
9
Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.延伸因子eEF2和真核80S核糖体的结构域运动促进tRNA易位。
EMBO J. 2004 Mar 10;23(5):1008-19. doi: 10.1038/sj.emboj.7600102. Epub 2004 Feb 19.
10
The Ribosome Assembly Factor Reh1 is Released from the Polypeptide Exit Tunnel in the Pioneer Round of Translation.核糖体组装因子Reh1在翻译的起始轮次中从多肽出口通道释放。
bioRxiv. 2023 Oct 23:2023.10.23.563604. doi: 10.1101/2023.10.23.563604.

引用本文的文献

1
Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states.利用cryoPRISM在细胞提取物中捕获核糖体结构：一种无需纯化的冷冻电镜方法揭示了新的结构状态。
bioRxiv. 2025 Aug 22:2025.08.21.669550. doi: 10.1101/2025.08.21.669550.
2
Trioxane-based MS-cleavable cross-linking mass spectrometry for profiling multimeric interactions of cellular networks.基于三聚甲醛的可质谱裂解交联质谱法用于分析细胞网络的多聚体相互作用。
Nat Commun. 2025 Jul 1;16(1):5585. doi: 10.1038/s41467-025-60642-3.

本文引用的文献

1
Translation machinery: the basis of translational control.翻译机制：翻译调控的基础。
J Genet Genomics. 2024 Apr;51(4):367-378. doi: 10.1016/j.jgg.2023.07.009. Epub 2023 Aug 1.
2
TORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes.TORC1 磷酸化并抑制核糖体保护因子 Stm1 以激活休眠核糖体。
EMBO J. 2023 Mar 1;42(5):e112344. doi: 10.15252/embj.2022112344. Epub 2023 Jan 24.
3
A molecular network of conserved factors keeps ribosomes dormant in the egg.一个保守因子的分子网络使卵中的核糖体处于休眠状态。
Nature. 2023 Jan;613(7945):712-720. doi: 10.1038/s41586-022-05623-y. Epub 2023 Jan 18.
4
Ribosome biogenesis and the cellular energy economy.核糖体生物发生与细胞能量代谢。
Curr Biol. 2022 Jun 20;32(12):R611-R617. doi: 10.1016/j.cub.2022.04.083.
5
CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.无假尿苷核糖体的冷冻电镜结构表明化学修饰对核糖体构象的影响。
Structure. 2022 Jul 7;30(7):983-992.e5. doi: 10.1016/j.str.2022.04.002. Epub 2022 Apr 29.
6
The space between notes: emerging roles for translationally silent ribosomes.注释之间的空间：翻译沉默核糖体的新兴作用。
Trends Biochem Sci. 2022 Jun;47(6):477-491. doi: 10.1016/j.tibs.2022.02.003. Epub 2022 Mar 1.
7
High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation.热嗜酸性真核 80S 核糖体的高分辨率结构揭示了转位的原子细节。
Nat Commun. 2022 Jan 25;13(1):476. doi: 10.1038/s41467-022-27967-9.
8
Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP.时间分辨冷冻电镜可视化核糖体与 EF-G 和 GTP 的转位。
Nat Commun. 2021 Dec 13;12(1):7236. doi: 10.1038/s41467-021-27415-0.
9
Ribo-Seq and RNA-Seq of ( ) and ( ) knockout yeast strains.（ ）和（ ）敲除酵母菌株的核糖体 RNA-Seq 和 RNA-Seq。
F1000Res. 2021 Nov 16;10:1162. doi: 10.12688/f1000research.74727.1. eCollection 2021.
10
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources.酿酒酵母基因组数据库的新数据和新合作：更新的参考基因组、等位基因和基因组资源联盟。
Genetics. 2022 Apr 4;220(4). doi: 10.1093/genetics/iyab224.