从内生真菌菌株HDS-Z-1E中分离得到的代谢产物及其对过氧化氢酶的激活作用，该内生真菌菌株从……分离得到 （原文此处“from”后缺少具体内容）
Metabolites isolated from HDS-Z-1E, an endophytic fungal strain isolated from and their activation effect of catalase.
作者信息
Tang Chuan, Gao Rui, Tang Xiaoyuan, Zhang Yu, Feng Weixing, Feng Baomin, Lu Xuan
机构信息
College of Life and Health, Dalian University, Dalian 116622, China.
出版信息
Chin Herb Med. 2023 May 22;16(2):227-230. doi: 10.1016/j.chmed.2022.12.009. eCollection 2024 Apr.
OBJECTIVE
To study the compounds isolated from HDS-Z-1E, an endophytic fungal strain isolated from and their activation effect of catalase (CAT).
METHODS
The chemical constituents were isolated from HDS-Z-1E, by using silica gel, Sephadex LH-20 and HPLC. The structural elucidations of five metabolites were elucidated on the basis of spectroscopic including H-NMR, C-NMR, HMBC and HSQC. Their activation sites of catalase have been investigated by molecular docking.
RESULTS
Five metabolites, compounds (-) were isolated from HDS-Z-1E and identified as 4-hydroxy-4-methyltetrahydro-2-pyran-2-one (), 4-hydroxymethyl-5, 6-dihydro-pyran-2-one (), 5, 6-dihydro-2-oxo-2-pyran-4-carboxylic (), -acetyl-hydrazinobenzoic acid (), and methyl 2-(2, 5-dihydroxyphenyl) acetate ().
CONCLUSION
Compound is a new compound. Compounds and may have potential activators of catalase, providing a theoretical basis for the development of CAT activators.
目的
研究从HDS-Z-1E（一种从[具体来源未给出]分离得到的内生真菌菌株）中分离出的化合物及其对过氧化氢酶（CAT）的激活作用。
方法
采用硅胶柱色谱、葡聚糖凝胶LH-20柱色谱和高效液相色谱法从HDS-Z-1E中分离化学成分。基于包括氢核磁共振（H-NMR）、碳核磁共振（C-NMR）、异核多键相关谱（HMBC）和异核单量子相干谱（HSQC）等光谱对5种代谢产物进行结构解析。通过分子对接研究它们对过氧化氢酶的激活位点。
结果
从HDS-Z-1E中分离得到5种代谢产物，化合物（-）被鉴定为4-羟基-4-甲基四氢-2-吡喃酮（）、4-羟甲基-5,6-二氢-2-吡喃酮（）、5,6-二氢-2-氧代-2-吡喃-4-羧酸（）、-乙酰肼基苯甲酸（）和2-(2,5-二羟基苯基)乙酸甲酯（）。
结论
化合物是一种新化合物。化合物和可能是过氧化氢酶的潜在激活剂，为开发CAT激活剂提供了理论依据。
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