Benčina Metka, Rawat Niharika, Paul Domen, Kovač Janez, Lakota Katja, Žigon Polona, Kralj-Iglič Veronika, Iglič Aleš, Junkar Ita
Department of Surface Engineering, Joz̆ef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia.
ACS Omega. 2024 Apr 16;9(17):19566-19577. doi: 10.1021/acsomega.4c01191. eCollection 2024 Apr 30.
The present study introduces an advanced surface modification approach combining electrochemical anodization and non-thermal plasma treatment, tailored for biomedical applications on stainless steel grade 316L (SS316L) surfaces. Nanopores with various diameters (100-300 nm) were synthesized with electrochemical anodization, and samples were further modified with non-thermal oxygen plasma. The surface properties of SS316L surfaces were examined by scanning electron microscopy, atomic force microscopy, X-ray photoemission spectroscopy, and Water contact angle measurements. It has been shown that a combination of electrochemical anodization and plasma treatment significantly alters the surface properties of SS316L and affects its interactions with blood platelets and human coronary cells. Optimal performance is attained on the anodized specimen featuring pores within the 150-300 nm diameter range, subjected to subsequent oxygen plasma treatment; the absence of platelet adhesion was observed. At the same time, the sample demonstrated good endothelialization and a reduction in smooth muscle cell adhesion compared to the untreated SS316L and the sample with smaller pores (100-150 nm). This novel surface modification strategy has significant implications for improving biocompatibility and performance of SS316L in biomedical applications.
本研究介绍了一种先进的表面改性方法，该方法将电化学阳极氧化和非热等离子体处理相结合，专门针对316L不锈钢（SS316L）表面的生物医学应用。通过电化学阳极氧化合成了具有不同直径（100 - 300纳米）的纳米孔，并用非热氧等离子体对样品进行了进一步改性。通过扫描电子显微镜、原子力显微镜、X射线光电子能谱和水接触角测量对SS316L表面的性能进行了检测。结果表明，电化学阳极氧化和等离子体处理相结合显著改变了SS316L的表面性能，并影响其与血小板和人冠状动脉细胞的相互作用。在直径范围为150 - 300纳米的多孔阳极氧化试样上，经过后续的氧等离子体处理后可获得最佳性能，未观察到血小板粘附现象。同时，与未处理的SS316L和孔径较小（100 - 150纳米）的样品相比，该样品表现出良好的内皮化，平滑肌细胞粘附减少。这种新型表面改性策略对于提高SS316L在生物医学应用中的生物相容性和性能具有重要意义。
