Crop Research Institute, Drnovska 507/73, Ruzyne, Czechia.
Department of Protection, Potato Research Institute Havlickuv Brod, Ltd., Havlickuv Brod, Czechia.
PLoS One. 2024 May 6;19(5):e0303238. doi: 10.1371/journal.pone.0303238. eCollection 2024.
The Colorado potato beetle (CPB; Leptinotarsa decemlineata) is an important potato pest with known resistance to pyrethroids and organophosphates in Czechia. Decreased efficacy of neonicotinoids has been observed in last decade. After the restriction of using chlorpyrifos, thiacloprid and thiamethoxam by EU regulation, growers seek for information about the resistance of CPB to used insecticides and recommended antiresistant strategies. The development of CPB resistance to selected insecticides was evaluated in bioassays in 69 local populations from Czechia in 2017-2022 and in 2007-2022 in small plot experiments in Zabcice in South Moravia. The mortality in each subpopulation in the bioassays was evaluated at the field-recommended rates of insecticides to estimate the 50% and 90% lethal concentrations (LC50 and LC90, respectively). High levels of CPB resistance to lambda-cyhalothrin and chlorpyrifos were demonstrated throughout Czechia, without significant changes between years and regions. The average mortality after application of the field-recommended rate of lambda-cyhalothrin was influenced by temperature before larvae were sampled for bioassays and decreased with increasing temperature in June. Downwards trends in the LC90 values of chlorpyrifos and the average mortality after application of the field-recommended rate of acetamiprid in the bioassay were recorded over a 6-year period. The baseline LC50 value (with 95% confidence limit) of 0.04 mg/L of chlorantraniliprole was established for Czech populations of CPBs for the purpose of resistance monitoring in the next years. Widespread resistance to pyrethroids, organophosphates and neonicotinoids was demonstrated, and changes in anti-resistant strategies to control CPBs were discussed.
科罗拉多马铃薯甲虫（CPB；Leptinotarsa decemlineata）是一种重要的马铃薯害虫，已知在捷克对拟除虫菊酯和有机磷具有抗性。在过去十年中，新烟碱类的效果有所下降。在欧盟法规限制使用毒死蜱、噻虫啉和噻虫嗪后，种植者寻求有关 CPB 对已用杀虫剂的抗性以及推荐的抗药性策略的信息。2017-2022 年在捷克的 69 个当地种群中以及 2007-2022 年在南摩拉维亚的 Zabcice 进行的小规模田间试验中，评估了 CPB 对选定杀虫剂的抗药性发展。在生物测定中，根据田间推荐的杀虫剂剂量评估每个亚种群的死亡率，以估计 50%和 90%致死浓度（LC50 和 LC90）。整个捷克都证明 CPB 对氯氟氰菊酯和毒死蜱具有高水平的抗性，且在年份和地区之间没有显著变化。在幼虫被用于生物测定之前，施药前的平均死亡率受温度的影响，并且在 6 月份随着温度的升高而降低。在 6 年期间，氯氟氰菊酯的 LC90 值和生物测定中施药推荐剂量后的平均死亡率呈下降趋势。为了在未来几年进行抗药性监测，建立了捷克 CPB 种群对氯虫苯甲酰胺的基线 LC50 值（置信限 95%）为 0.04mg/L。证明了对拟除虫菊酯、有机磷和新烟碱类的广泛抗性，并讨论了控制 CPB 的抗药性策略的变化。
