类风湿关节炎患者血液、骨髓及滑膜单个核细胞产生IgM类风湿因子的情况。

IgM rheumatoid factor elaboration by blood, bone marrow, and synovial mononuclear cells in patients with rheumatoid arthritis.

作者信息

Panush R S, Bittner A K, Sullivan M, Katz P, Longley S

出版信息

Clin Immunol Immunopathol. 1985 Mar;34(3):387-91. doi: 10.1016/0090-1229(85)90187-4.

Abstract

IgM rheumatoid factor (RF) elaboration by rheumatoid arthritis (RA) synovial, bone marrow, and blood mononuclear cells (MNC) is reported. IgM RF was prepared from RF-positive sera by sequential euglobulin precipitation, Sephacryl S300 gel filtration, and IgG-Sepharose affinity chromatography. Purified material, which contained no detectable IgG or IgA, was used in an enzyme-linked immunosorbent assay (ELISA) to quantitate cellular elaboration of IgM RF. Excellent standard curves (r2 = 0.98) were obtained without nonspecific binding of samples or antisera to IgG-coated microtiter plates and without cross-reactivity of standards with antisera other than anti-IgM. We found RA blood MNC (11 patients) spontaneously averaged 15 ng/ml IgM RF (6% of total IgM produced), but elaborated 254 ng/ml IgM RF following pokeweed mitogen (PWM) stimulation (22 patients), exceeding that of 13 normal controls. Bone marrow MNC spontaneously (4 patients) produced 71 ng/ml IgM RF and secreted 78 ng/ml IgM RF with PWM stimulation (9 patients). In contrast synovial fluid MNC (5 patients) spontaneously elaborated 6652 ng/ml IgM RF, significantly (P less than 0.05) more than blood or bone marrow MNC; PWM-stimulated synovial fluid MNC (5 patients) produced 5472 ng/ml IgM RF. These observations confirm selective localization of activated, IgM RF-producing cells to the rheumatoid synovial space.

摘要

据报道，类风湿关节炎（RA）的滑膜、骨髓和血液单核细胞（MNC）可产生IgM类风湿因子（RF）。通过依次进行优球蛋白沉淀、Sephacryl S300凝胶过滤和IgG-琼脂糖亲和层析，从RF阳性血清中制备IgM RF。将不含可检测到的IgG或IgA的纯化物质用于酶联免疫吸附测定（ELISA），以定量IgM RF的细胞产生情况。获得了出色的标准曲线（r2 = 0.98），样品或抗血清与IgG包被的微量滴定板无非特异性结合，且标准品与抗IgM以外的抗血清无交叉反应。我们发现，RA血液MNC（11例患者）自发产生的IgM RF平均为15 ng/ml（占总产生IgM的6%），但在美洲商陆有丝分裂原（PWM）刺激后（22例患者）产生254 ng/ml IgM RF，超过了13名正常对照者。骨髓MNC（4例患者）自发产生71 ng/ml IgM RF，在PWM刺激下（9例患者）分泌78 ng/ml IgM RF。相比之下，滑膜液MNC（5例患者）自发产生6652 ng/ml IgM RF，显著高于血液或骨髓MNC（P小于0.05）；PWM刺激的滑膜液MNC（5例患者）产生5472 ng/ml IgM RF。这些观察结果证实了活化的、产生IgM RF的细胞选择性定位于类风湿滑膜间隙。