Spitz M, Gearing A, Callus M, Spitz L, Thorpe R
Immunology. 1985 Mar;54(3):527-32.
In this report, we describe experiments which demonstrate that antigenic stimulation in vivo causes the appearance of cells in both spleen and lymph node which secrete interleukin-2 (IL-2). Cells also appear in these organs which proliferate in response to IL-2. For these experiments, sheep red cells (SRBC) were injected into the spleens or footpads of mice, and cell suspensions from spleens or popliteal lymph nodes prepared at various times after antigenic stimulation. These cells were assayed for their ability to respond to IL-2, and their cell culture supernatants for secreted IL-2. The proliferative response to IL-2 steadily increased following SRBC injection to reach a peak at Day 2 for spleen cells and at Day 3 for lymph node cells. Maximal production of IL-2 was displaced from the maximal response to the lymphokine by peaking one day later for both organs. Our results strongly implicate the participation of IL-2 in the in vivo immune response and suggest the existence of in vivo regulatory mechanisms, which can control the time of IL-2 production and also the appearance of cells with receptors for IL-2.
在本报告中，我们描述了一些实验，这些实验表明体内的抗原刺激会导致脾脏和淋巴结中出现分泌白细胞介素-2（IL-2）的细胞。这些器官中也会出现对IL-2产生增殖反应的细胞。在这些实验中，将绵羊红细胞（SRBC）注射到小鼠的脾脏或足垫中，并在抗原刺激后的不同时间制备脾脏或腘窝淋巴结的细胞悬液。检测这些细胞对IL-2的反应能力，以及它们的细胞培养上清液中分泌的IL-2。注射SRBC后，对IL-2的增殖反应稳步增加，脾脏细胞在第2天达到峰值，淋巴结细胞在第3天达到峰值。两个器官中IL-2的最大产量比淋巴因子的最大反应延迟一天达到峰值。我们的结果有力地表明IL-2参与了体内免疫反应，并提示存在体内调节机制，该机制可以控制IL-2的产生时间以及具有IL-2受体的细胞的出现。
