Gullberg M, Ivars F, Coutinho A, Larsson E L
J Immunol. 1981 Aug;127(2):407-11.
The detailed kinetics of TCGF accumulation in Con A-stimulated spleen cell cultures shows a maximum at 24 hr, with a subsequent decrease in activity. This decrease is not due to the appearance of inhibitory substances "masking" TCGF activity. Pulse experiments show that the rate of TCGF production falls sharply after 18 hr and is completely arrested after 24 hr of Con A stimulation. The arrest in TCGF production is the result neither of culture depletion in medium components nor of limiting accessory cell function or inactivation of the lectin, and it thus seem to be the result of inactivation of TCGF-producing T cells. This regulation is not the result of a TCGF-mediated feedback mechanism but rather of lectin-induced suppressive cells that appear in culture after 24 hr and turn off de novo production of TCGF in fresh cultures.
在伴刀豆球蛋白A刺激的脾细胞培养物中，TCGF积累的详细动力学显示在24小时达到最大值，随后活性下降。这种下降不是由于出现了“掩盖”TCGF活性的抑制物质。脉冲实验表明，在伴刀豆球蛋白A刺激18小时后，TCGF的产生速率急剧下降，在24小时后完全停止。TCGF产生的停止既不是培养基成分中培养物耗尽的结果，也不是辅助细胞功能受限或凝集素失活的结果，因此似乎是产生TCGF的T细胞失活的结果。这种调节不是TCGF介导的反馈机制的结果，而是凝集素诱导的抑制细胞的结果，这些抑制细胞在24小时后出现在培养物中，并在新鲜培养物中关闭TCGF的从头产生。
