Hirose T, Hara M, Kitani A, Hirose W, Norioka K, Kawagoe M, Nakamura H
Scand J Immunol. 1985 Feb;21(2):141-50. doi: 10.1111/j.1365-3083.1985.tb01413.x.
We examined the production of and the response to B-cell growth factor (BCGF) and B-cell differentiation factor (BCDF) in 21 patients with systemic lupus erythematosus (SLE) and 23 normal subjects. T cells, 2.5 X 10(6)/ml, were cultured for 24 or 72 h with 1% phytohaemagglutinin (PHA). After absorption of PHA by chicken erythrocytes (CRBC), they were used for BCGF and BCDF. In inactive SLE, BCGF activity was significantly lower than that in normal subjects. Active SLE contained two separate groups, one showing normal BCGF activity and the other showing lower activity than normal. In contrast, BCDF activity from initial culture in active SLE was elevated. The B-cell response both to BCGF and BCDF was elevated in active SLE without Staphylococcus aureus Cowan I antigen (SAC) preactivation. However, the B-cell response to SAC was markedly disturbed. Thus SLE B cells were shifted to the mature state in vivo. We also demonstrated pivotal abnormalities of monocytes in SLE B-cell growth and differentiation. These results may contribute to the understanding of the abnormalities of T-B interactions and the overproduction of antibody in SLE.
我们检测了21例系统性红斑狼疮（SLE）患者和23名正常受试者体内B细胞生长因子（BCGF）及B细胞分化因子（BCDF）的产生情况和反应。将浓度为2.5×10⁶/ml的T细胞与1%植物血凝素（PHA）一起培养24或72小时。在用鸡红细胞（CRBC）吸收PHA后，将其用于检测BCGF和BCDF。在非活动期SLE中，BCGF活性显著低于正常受试者。活动期SLE患者分为两组，一组BCGF活性正常，另一组活性低于正常水平。相比之下，活动期SLE初始培养的BCDF活性升高。在未用金黄色葡萄球菌Cowan I抗原（SAC）预激活的活动期SLE中，B细胞对BCGF和BCDF的反应均增强。然而，B细胞对SAC的反应明显受损。因此，SLE中的B细胞在体内已转变为成熟状态。我们还证明了SLE中单核细胞在B细胞生长和分化过程中存在关键异常。这些结果可能有助于理解SLE中T - B相互作用异常及抗体过度产生的机制。
