Sadeh Pegah, Zeinali Sedigheh, Rastegari Banafsheh, Najafipour Iman
Department of Nanochemical Engineering, School of Advanced Technologies, Shiraz University, Iran.
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Science, Shiraz, Iran.
Heliyon. 2024 Apr 25;10(9):e30349. doi: 10.1016/j.heliyon.2024.e30349. eCollection 2024 May 15.
Beta-cyclodextrin Metal-Organic Framework (β-CD-MOF) is a unique class of porous materials that merges the inherent properties of cyclodextrins with the structural advantages of metal-organic frameworks (MOFs). When combined with the concept of MOFs, which are crystalline structures composed of metal ions or clusters linked by organic ligands, the resulting β-CD-MOF holds immense potential for various applications, especially in the field of drug delivery. In this study, biocompatible metal-organic frameworks (MOFs) synthesized using β-Cyclodextrin (β-CD) and potassium enabled drug delivery of curcumin (CCM) to cancerous cells. Functionalizing β-CD-MOF with l-glutamine (glutamine-β-CD-MOF) enhanced cancer cell-specific targeting due to glutamine's essential role in cancer cell proliferation and energy pathways. Amino group functionalization provided further functionalization opportunities. Gelatin coating (gelatin@β-CD-MOF) facilitated controlled drug release in an acidic medium. High drug loading capacities (52.38-55.63 %) were achieved for β-CD-MOF@CCM and glutamine-β-CD-MOF@CCM, leveraging the high porosity and affinity of amine and phenol groups of curcumin. The MTT assay highlighted the specificity and differentiation of glutamine-β-CD-MOF in targeting cancerous over normal cells. These functionalized β-CD MOFs efficiently encapsulate curcumin, ensuring controlled drug release and enhanced therapeutic efficacy, particularly in cancer therapy.
β-环糊精金属有机框架（β-CD-MOF）是一类独特的多孔材料，它将环糊精的固有特性与金属有机框架（MOF）的结构优势相结合。当与由金属离子或簇通过有机配体连接而成的晶体结构MOF的概念相结合时，所得的β-CD-MOF在各种应用中具有巨大潜力，尤其是在药物递送领域。在本研究中，使用β-环糊精（β-CD）和钾合成的生物相容性金属有机框架（MOF）实现了姜黄素（CCM）向癌细胞的药物递送。用L-谷氨酰胺对β-CD-MOF进行功能化（谷氨酰胺-β-CD-MOF）增强了癌细胞特异性靶向，因为谷氨酰胺在癌细胞增殖和能量途径中起着重要作用。氨基功能化提供了进一步的功能化机会。明胶包被（明胶@β-CD-MOF）促进了在酸性介质中的控释。利用姜黄素胺基和酚基的高孔隙率和亲和力，β-CD-MOF@CCM和谷氨酰胺-β-CD-MOF@CCM实现了高载药量（52.38 - 55.63%）。MTT试验突出了谷氨酰胺-β-CD-MOF在靶向癌细胞而非正常细胞方面的特异性和区分性。这些功能化的β-CD MOF有效地包裹姜黄素，确保控释并提高治疗效果，特别是在癌症治疗中。
