联合血清CTRP7和CTRP15水平作为胰岛素抵抗和2型糖尿病的新型生物标志物。
Combined serum CTRP7 and CTRP15 levels as a novel biomarker for insulin resistance and type 2 diabetes mellitus.
作者信息
Xue Shiyao, Ling Jiaxiu, Tian Mingyuan, Li Ke, Li Shengbing, Liu Dongfang, Li Ling, Yang Mengliu, Yang Gangyi
机构信息
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Key Laboratory of Diagnostic Medicine (Ministry of Education) and Department of Clinical Biochemistry, College of Laboratory Medicine, Chongqing Medical University, China.
出版信息
Heliyon. 2024 Apr 29;10(9):e30029. doi: 10.1016/j.heliyon.2024.e30029. eCollection 2024 May 15.
AIMS
This study aimed to examine the alterations in the serum CTRP7 and CTRP15 concentrations in patients newly diagnosed with type 2 diabetes mellitus (T2DM) and to assess the diagnostic potential of the log10 (CTRP7+CTRP15) for insulin resistance (IR) and T2DM.
METHODS
Serum CTRP7, CTRP15, and adiponectin levels were measured using an enzyme-linked immunosorbent assay (ELISA). Bioinformatics analysis was conducted to investigate CTRP7 and CTRP15-related genes and metabolic signaling pathways.
RESULTS
Log10 (CTRP7+CTRP15) levels were notably elevated in the impaired glucose tolerance (IGT) and T2DM cohorts compared with those in the normal control (NGT) cohort. Log10(CTRP7+CTRP15) exhibited positive correlations with HOMA-IR, area under the glucose curve (AUCg), HbA1c%, triglyceride (TG), visceral adiposity index (VAI), body mass index (BMI), and free fatty acid (FFA), levels but negative correlations with adiponectin. Multivariate stepwise regression analysis revealed that HOMA-IR, BMI, HbA1c and FFA levels were independent factors affecting the log10 (CTRP7+CTRP15). Logistic regression analysis revealed that log10 (CTRP7+CTRP15) was independently associated with T2DM and significantly associated with increased risk. Receiver operating characteristic (ROC) curve analysis indicated that the predictive value of log10 (CTRP7+CTRP15) for T2DM and IR was superior to that of CTRP7 or CTRP15 alone. Intervention studies demonstrated that insulin, FFAs and acute exercise contribute to the elevation of serum CTRP7 levels, while hyperglycemia inhibited CTRP7 secretion. Short-term changes in blood glucose, insulin, FFA and acute exercise had minimal effects on serum CTRP15 levels. Bioinformatics analysis revealed that CTRP7 and CTRP15 interact with multiple metabolism-related genes and are enriched in glucose and lipid metabolism-related pathways.
CONCLUSION
Log10 (CTRP7+CTRP15) may serve as a valuable diagnostic marker for the management of metabolic-related diseases, particularly T2DM and IR.
目的
本研究旨在检测新诊断的2型糖尿病（T2DM）患者血清CTRP7和CTRP15浓度的变化，并评估log10（CTRP7 + CTRP15）对胰岛素抵抗（IR）和T2DM的诊断潜力。
方法
采用酶联免疫吸附测定（ELISA）法检测血清CTRP7、CTRP15和脂联素水平。进行生物信息学分析以研究CTRP7和CTRP15相关基因及代谢信号通路。
结果
与正常对照（NGT）队列相比，糖耐量受损（IGT）和T2DM队列中的log10（CTRP7 + CTRP15）水平显著升高。Log10（CTRP7 + CTRP15）与稳态模型评估胰岛素抵抗（HOMA-IR）、葡萄糖曲线下面积（AUCg）、糖化血红蛋白（HbA1c）％、甘油三酯（TG）、内脏脂肪指数（VAI）、体重指数（BMI）和游离脂肪酸（FFA）水平呈正相关，但与脂联素呈负相关。多因素逐步回归分析显示，HOMA-IR、BMI、HbA1c和FFA水平是影响log10（CTRP7 + CTRP15）的独立因素。逻辑回归分析显示，log10（CTRP7 + CTRP15）与T2DM独立相关且与风险增加显著相关。受试者工作特征（ROC）曲线分析表明，log10（CTRP7 + CTRP15）对T2DM和IR的预测价值优于单独的CTRP7或CTRP15。干预研究表明，胰岛素、FFA和急性运动导致血清CTRP7水平升高,而高血糖抑制CTRP7分泌。血糖、胰岛素、FFA和急性运动的短期变化对血清CTRP15水平影响最小。生物信息学分析显示，CTRP7和CTRP15与多个代谢相关基因相互作用，并富集于葡萄糖和脂质代谢相关途径。
结论
Log10（CTRP7 + CTRP15）可能作为代谢相关疾病，特别是T2DM和IR管理的有价值诊断标志物。
Zotero 插件