Solorio-Rodriguez Silvia Aidee, Wu Dongmei, Boyadzhiev Andrey, Christ Callum, Williams Andrew, Halappanavar Sabina
Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON K1A0K9, Canada.
Department of Biology, University of Ottawa, Ottawa, ON K1N6N5, Canada.
Nanomaterials (Basel). 2024 Apr 24;14(9):743. doi: 10.3390/nano14090743.
Metal oxide nanoparticles (MONP/s) induce DNA damage, which is influenced by their physicochemical properties. In this study, the high-throughput CometChip and micronucleus (MicroFlow) assays were used to investigate DNA and chromosomal damage in mouse lung epithelial cells induced by nano and bulk sizes of zinc oxide, copper oxide, manganese oxide, nickel oxide, aluminum oxide, cerium oxide, titanium dioxide, and iron oxide. Ionic forms of MONPs were also included. The study evaluated the impact of solubility, surface coating, and particle size on response. Correlation analysis showed that solubility in the cell culture medium was positively associated with response in both assays, with the nano form showing the same or higher response than larger particles. A subtle reduction in DNA damage response was observed post-exposure to some surface-coated MONPs. The observed difference in genotoxicity highlighted the mechanistic differences in the MONP-induced response, possibly influenced by both particle stability and chemical composition. The results highlight that combinations of properties influence response to MONPs and that solubility alone, while playing an important role, is not enough to explain the observed toxicity. The results have implications on the potential application of read-across strategies in support of human health risk assessment of MONPs.
金属氧化物纳米颗粒（MONP/s）会引发DNA损伤，这受到其物理化学性质的影响。在本研究中，采用高通量彗星芯片和微核（MicroFlow）检测法，来研究纳米级和块状的氧化锌、氧化铜、氧化锰、氧化镍、氧化铝、氧化铈、二氧化钛和氧化铁对小鼠肺上皮细胞造成的DNA和染色体损伤。MONP的离子形式也在研究范围内。该研究评估了溶解度、表面涂层和粒径对细胞反应的影响。相关性分析表明，在两种检测中，细胞培养基中的溶解度均与细胞反应呈正相关，纳米形式的反应与较大颗粒相同或更高。暴露于某些表面涂层的MONP后，观察到DNA损伤反应略有降低。观察到的遗传毒性差异突出了MONP诱导反应的机制差异，这可能受颗粒稳定性和化学成分两者的影响。结果表明，多种性质的组合会影响对MONP的反应，仅溶解度虽起着重要作用，但不足以解释所观察到的毒性。这些结果对类推策略在支持MONP人类健康风险评估中的潜在应用具有启示意义。
