Weidner N, McDonald J M, Tieber V L, Smith C H, Kessler G, Ladenson J H, Dietzler D N
Clin Chim Acta. 1979 Sep 15;97(1):9-17. doi: 10.1016/0009-8981(79)90019-6.
The EMIT technique for theophylline measurement as adapted to the Abbott Bichromatic Analyzer (ABA-100), was compared to two other established procedures. EMIT results correlated well with those obtained with high pressure liquid chromatographic (HPLC) (r = 0.995, N = 54) and spectrophotometric (UV) (r = 0.95, N = 37) methods when the patient samples used were free of apparent interferences in the comparison methods. On samples exhibiting interferences with HPLC and/or UV methods, the EMIT assay gave the same results as a gas-liquid chromatographic procedure which showed no interferences. In addition, theophylline metabolites, xanthine analogs and many commonly administered drugs did not interfere with the EMIT theophylline assay. An interassay coefficient of variation of 3.5--6.5% was demonstrated. Our data indicate that the EMIT technique exhibits adequate precision and specificity for routine theophylline determinations. Unlike the HPLC or UV procedures, we have not encountered any interferences with the EMIT technique.
将适用于雅培双色分析仪（ABA - 100）的茶碱测量发射法与另外两种既定方法进行了比较。当所使用的患者样本在比较方法中无明显干扰时，发射法结果与高压液相色谱法（HPLC）（r = 0.995，N = 54）和分光光度法（UV）（r = 0.95，N = 37）所获结果相关性良好。对于显示出对HPLC和/或UV方法有干扰的样本，发射法测定结果与未显示干扰的气液色谱法结果相同。此外，茶碱代谢物、黄嘌呤类似物和许多常用药物均不干扰发射法茶碱测定。检测到批间变异系数为3.5 - 6.5%。我们的数据表明，发射法技术在常规茶碱测定中具有足够的精密度和特异性。与HPLC或UV方法不同，我们未遇到发射法技术的任何干扰。
