Kampa I S, Dunikoski L K, Jarzabek J I, Grubesich D
Ther Drug Monit. 1979;1(2):249-56. doi: 10.1097/00007691-197901020-00006.
We examined 50 serum samples, submitted to our laboratories for theophylline levels, by the Enzyme Multiplied Immuno Technique (EMIT), by a radioimmunoassay procedure, and by a high pressure liquid chromatographic assay. All three procedures classified patients similarly into subtherapeutic, therapeutic, and toxic levels. Precision and recovery values showed no significant differences between the three procedures. Precision ranged from 3.24 to 13.66%, while recovery values were from 98.6 to 110.0%. Significant differences were observed in the cost per assay and rate of throughput. The EMIT procedure was most costly and required the greatest amount of time. The excellent correlation between the Syva EMIT assay and the other two procedures makes this a desirable method for those laboratories which lack the more expensive instruments required for the other two procedures.
我们采用酶放大免疫技术（EMIT）、放射免疫分析法和高压液相色谱分析法，对提交至我们实验室检测茶碱水平的50份血清样本进行了检测。所有这三种方法对患者的分类结果相似，可分为治疗水平以下、治疗水平和中毒水平。三种方法的精密度和回收率值无显著差异。精密度范围为3.24%至13.66%，回收率值为98.6%至110.0%。在每次检测的成本和通量率方面观察到显著差异。EMIT方法成本最高，所需时间最长。Syva EMIT检测法与其他两种方法之间的良好相关性，使其成为那些缺乏其他两种方法所需更昂贵仪器的实验室的理想选择。
