Boobis S, Persaud N, Richens A
Ther Drug Monit. 1979;1(2):257-63. doi: 10.1097/00007691-197901020-00007.
Twenty-eight laboratories in the United Kingdom, Europe, and Australia participated in pilot study to assess interlaboratory variability in determining serum theophylline levels. Each laboratory received two samples of pooled serum containing theophylline each month and was asked to report their results and method to us. Data obtained over a period of 12 months were collated, enabling certain conclusions to be drawn. Five methods of analysis were used: high pressure liquid chromatography (HPLC), gas--liquid chromatography, EMIT, thin-layer chromatography, and ultraviolet spectroscopy (UV). All of these methods, with the exception of UV, were found to be satisfactory, with HPLC marginally superior. Several laboratories improved their assay techniques over the period of operation of the scheme, and all found the scheme helpful in assessing the performance of their methods.
英国、欧洲和澳大利亚的28个实验室参与了一项试点研究，以评估测定血清茶碱水平时实验室间的差异。每个实验室每月收到两份含茶碱的混合血清样本，并被要求向我们报告其结果和方法。对12个月期间获得的数据进行了整理，从而得出了某些结论。使用了五种分析方法：高压液相色谱法（HPLC）、气液色谱法、酶放大免疫测定技术（EMIT）、薄层色谱法和紫外光谱法（UV）。除紫外光谱法外，所有这些方法都被认为是令人满意的，其中高压液相色谱法略胜一筹。在该计划实施期间，几个实验室改进了其检测技术，并且所有实验室都发现该计划有助于评估其方法的性能。
