Department of Biotechnology & Medical Engineering, National Institute of Technology Rourkela, Odisha, 769008, India.
Arch Microbiol. 2024 May 12;206(6):258. doi: 10.1007/s00203-024-03968-8.
Phycocyanin, a blue-coloured pigment, predominantly found and derived from Spirulina sp., has gained researchers' interest due to its vibrant hues and other attractive properties like antioxidant and anti-microbial. However, the lack of reliable and sustainable phycocyanin extraction strategies without compromising the quality has hindered the scaling up of its production processes for commercial purposes. Here in this study, phycocyanin was extracted from wet and dry biomass Spirulina sp., using three different physical cell disruption methods (ultrasonication, homogenization, and freeze-thaw cycles) combined with two different buffers (phosphate buffer and acetate buffer) and water (as control). The result showed that the freeze-thaw method combined with acetate buffer produced the highest yield (25.013 ± 2.572 mg/100 mg) with a purity ratio of 0.806 ± 0.079. Furthermore, when subjected to 30% salt stress, 1.9 times higher phycocyanin yield with a purity ratio of 1.402 ± 0.609 was achieved using the previously optimized extraction method.
藻蓝蛋白是一种蓝色色素，主要存在于螺旋藻中，并从螺旋藻中提取得到，由于其鲜艳的色调和抗氧化、抗微生物等其他有吸引力的特性，引起了研究人员的兴趣。然而，由于缺乏可靠和可持续的藻蓝蛋白提取策略，又不影响质量，因此限制了其生产过程的扩大，无法用于商业目的。在这项研究中，使用三种不同的物理细胞破碎方法（超声、匀浆和冻融循环）结合两种不同的缓冲液（磷酸盐缓冲液和醋酸盐缓冲液）和水（作为对照）从湿生物质和干生物质螺旋藻中提取藻蓝蛋白。结果表明，醋酸盐缓冲液结合冻融法的提取率最高（25.013 ± 2.572 mg/100 mg），纯度比为 0.806 ± 0.079。此外，在 30%盐胁迫下，使用先前优化的提取方法可获得 1.9 倍的藻蓝蛋白产量，纯度比为 1.402 ± 0.609。
