The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
Department of Clinical Laboratory Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China.
Front Cell Infect Microbiol. 2022 Jul 27;12:937864. doi: 10.3389/fcimb.2022.937864. eCollection 2022.
Recently, chronic obstructive pulmonary disease (COPD) has been considered as a common risk factor of non-small cell lung cancer (NSCLC). However, very few studies have been conducted on the effects of COPD on the lung microbiota in patients with NSCLC. To identify the lung microbiota in patients with COPD and NSCLC (CN), the microbiome of the induced sputa of 90 patients was analyzed using 16S rDNA sequencing. The results showed no significant differences in the bacterial diversities of induced sputa among patients with COPD, NSCLC, and CN and no intrinsic differences among patients with different pathological types of lung cancer. After surgical operation, the diversities of the induced sputa in patients with CN significantly decreased. More remarkably, both the microbial community phenotypes and the components of the induced sputa in patients with CN obviously differed from those in patients with COPD or NSCLC. The relative abundances of , , , and significantly decreased, but those of and significantly increased in patients with CN compared with those in patients with COPD or NSCLC alone, resulting in increased Gram-negative microbiota and, therefore, in potential pathogenicity and stress tolerance, as well as in enhancement of microbial glycolipid metabolism, amino acid metabolism, and oxidative stress. Although COPD did not affect the number of pulmonary flora species in patients with NSCLC, these significant alterations in the microbial populations, phenotypes, and functions of induced sputa due to COPD would contribute to inflammation-derived cancer progression in patients with CN.
最近，慢性阻塞性肺疾病（COPD）被认为是非小细胞肺癌（NSCLC）的一个常见危险因素。然而，关于 COPD 对 NSCLC 患者肺部微生物群的影响的研究甚少。为了鉴定 COPD 和 NSCLC 患者（CN）的肺部微生物群，使用 16S rDNA 测序分析了 90 例患者诱导痰的微生物组。结果表明，COPD、NSCLC 和 CN 患者诱导痰的细菌多样性无显著差异，不同肺癌病理类型患者之间也无内在差异。手术后，CN 患者的诱导痰多样性明显下降。更显著的是，CN 患者的微生物群落表型和诱导痰成分明显不同于 COPD 或 NSCLC 患者。与 COPD 或 NSCLC 患者相比，CN 患者的 、 、 、 和 相对丰度显著降低，而 和 相对丰度显著增加，导致革兰氏阴性菌增多，因此潜在的致病性和应激耐受性增强，以及微生物糖脂代谢、氨基酸代谢和氧化应激增强。虽然 COPD 不影响 NSCLC 患者肺部菌群的种类数量，但 COPD 导致的诱导痰微生物群的这些显著变化，表型和功能将有助于 CN 患者炎症衍生的癌症进展。
