Department of Pathobiology, Universidad de la República, Montevideo, Uruguay.
Veterinary Clinics & Hospital, Faculty of Veterinary, Universidad de la República, Montevideo, Uruguay.
Methods Mol Biol. 2024;2808:177-195. doi: 10.1007/978-1-0716-3870-5_14.
We describe the use of conventional histology and immunohistochemistry against canine distemper virus (CDV) to examine the brains of domestic dogs with a confirmed diagnosis of CDV infection. Histologically, to identify the main typical lesions, we used conventional H&E stain; to evaluate the progressive demyelination, we used Luxol Fast Blue stain; and to identify the presence of viral particles in these affected regions, we used immunohistochemistry against CDV. We confirm that the histopathological analysis of brains of distemper-infected dogs is a powerful tool to evaluate the typical brain lesions and could be used as an interesting natural model to continue studying the pathogenesis of canine distemper in different species and/or other morbillivirus infections, like measles.
我们描述了使用常规组织学和免疫组织化学方法检测犬瘟热病毒 (CDV)，以检查确诊为 CDV 感染的家犬的大脑。组织学上，为了识别主要的典型病变，我们使用了常规的 H&E 染色；为了评估进行性脱髓鞘，我们使用了卢戈氏碘液染色；为了识别这些受影响区域中的病毒颗粒，我们使用了针对 CDV 的免疫组织化学方法。我们证实，对感染犬瘟热病毒的犬的脑组织进行组织病理学分析是评估典型脑病变的有力工具，并且可以作为一个有趣的自然模型，用于在不同物种和/或其他副黏病毒感染（如麻疹）中继续研究犬瘟热的发病机制。
