Departments of Pathobiology, Veterinary Faculty, Universidad de la República, Montevideo, Uruguay.
Veterinary Clinics & Hospital, Veterinary Faculty, Universidad de la República, Montevideo, Uruguay.
J Vet Diagn Invest. 2021 Jul;33(4):640-647. doi: 10.1177/10406387211009210. Epub 2021 Apr 17.
We examined the cerebellum and cerebrum of 4 vaccinated dogs, 3-60-mo-old, that displayed clinical signs of canine distemper virus (CDV) infection, and died 7-40 d after developing neurologic signs. The main histologic lesions were demyelination, gliosis, meningitis, perivascular lymphocytic cuffing, and inclusion bodies. These lesions were similar in all 4 cases regardless of the time since vaccination, except that meningoencephalitis and gliosis were subacute in 3 dogs and chronic in 1 dog. However, these differences did not appear to be related to their vaccination status. Immunohistologically, a CDV-positive immunoreaction was seen mainly in astrocytes, neurons and their axons, lymphocytes around and in the blood vessels of the pia mater and choroid plexus, ependymal cells of each ventricle, and the cells of the choroid plexus. The histologic and immunohistologic changes were similar in the cerebellum and cerebrum. The genetic characterization of the virus strains in 2 of these naturally occurring canine distemper cases confirmed that they were South American wild-type strains (Kiki and Uy251) belonging to the EU1/SA1 lineage. These strains are not included in the commercial CDV vaccines available in Uruguay.
我们检查了 4 只接种疫苗的犬的小脑和大脑，这些犬年龄在 3-60 个月之间，出现了犬瘟热病毒 (CDV) 感染的临床症状，并在出现神经症状后 7-40 天死亡。主要的组织学病变是脱髓鞘、神经胶质增生、脑膜炎、血管周围淋巴细胞袖套和包涵体。这 4 个病例的所有病变都相似，与接种疫苗的时间无关，除了脑膜脑炎和神经胶质增生在 3 只犬中为亚急性，在 1 只犬中为慢性。然而，这些差异似乎与它们的疫苗接种状态无关。免疫组织化学检查显示，CDV 阳性反应主要见于星形胶质细胞、神经元及其轴突、围绕软脑膜和脉络丛血管的淋巴细胞、每个脑室的室管膜细胞和脉络丛的细胞。小脑和大脑的组织学和免疫组织化学变化相似。对这 2 例自然发生的犬瘟热病例中的病毒株进行的遗传特征分析证实，它们是属于 EU1/SA1 谱系的南美野生型株 (Kiki 和 Uy251)。这些毒株未包含在乌拉圭可获得的商业 CDV 疫苗中。
