Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea.
J Agric Food Chem. 2024 May 29;72(21):11871-11884. doi: 10.1021/acs.jafc.4c01650. Epub 2024 May 14.
Genome editing is a crucial technology for obtaining desired phenotypes in a variety of species, ranging from microbes to plants, animals, and humans. With the advent of CRISPR-Cas technology, it has become possible to edit the intended sequence by modifying the target recognition sequence in guide RNA (gRNA). By expressing multiple gRNAs simultaneously, it is possible to edit multiple targets at the same time, allowing for the simultaneous introduction of various functions into the cell. This can significantly reduce the time and cost of obtaining engineered microbial strains for specific traits. In this review, we investigate the resolution of multiplex genome editing and its application in engineering microorganisms, including bacteria and yeast. Furthermore, we examine how recent advancements in artificial intelligence technology could assist in microbial genome editing and engineering. Based on these insights, we present our perspectives on the future evolution and potential impact of multiplex genome editing technologies in the agriculture and food industry.
基因组编辑是一种在从微生物到植物、动物和人类等各种物种中获得所需表型的关键技术。随着 CRISPR-Cas 技术的出现，通过修改向导 RNA (gRNA)中的目标识别序列，已经可以编辑预期的序列。通过同时表达多个 gRNA，可以同时编辑多个靶标，从而可以将各种功能同时引入细胞。这可以显著减少获得具有特定特性的工程微生物菌株所需的时间和成本。在这篇综述中，我们研究了多重基因组编辑的分辨率及其在工程微生物，包括细菌和酵母中的应用。此外，我们还研究了人工智能技术的最新进展如何有助于微生物基因组编辑和工程。基于这些见解，我们提出了我们对未来农业和食品工业中多重基因组编辑技术的发展和潜在影响的看法。
