Harris J P, Woolf N K, Ryan A F
Am J Otolaryngol. 1985 May-Jun;6(3):148-52. doi: 10.1016/s0196-0709(85)80077-6.
The development of systemic humoral and cellular immunity following antigen presentation in the inner ear was compared with that seen following middle ear and peritoneal inoculation routes. Antibody developing against keyhole limpet hemocyanin was measured by a sensitive enzyme-linked immunofiltration assay, and cell-mediated immunity was measured by in vitro lymphocyte blastogenesis. The inner ear and peritoneal routes of antigen presentation resulted in a parallel rise in antibody over a 3-week period. In contrast, the middle ear route resulted in a weak, transient antibody response by 2 weeks. The acquisition of cell-mediated immunity occurred earliest (day 14) in the group receiving antigen intraperitoneally. A significant but smaller proliferative response was also seen in the group receiving antigen via the inner ear route on days 14 and 21. In contrast, the middle ear route failed to result in cell-mediated immunity. These studies indicate that the inner ear is an effective route of antigen processing which results in the acquisition of systemic humoral and cellular immunity. The development of systemic immunity, in turn, has been found to be protective of the inner ear.
将内耳抗原呈递后全身体液免疫和细胞免疫的发展与中耳和腹腔接种途径后的情况进行了比较。通过灵敏的酶联免疫过滤测定法检测针对钥孔戚血蓝蛋白产生的抗体，通过体外淋巴细胞增殖测定法检测细胞介导的免疫。内耳和腹腔抗原呈递途径在3周内导致抗体平行上升。相比之下，中耳途径在2周时产生微弱、短暂的抗体反应。接受腹腔内抗原的组最早（第14天）获得细胞介导的免疫。在第14天和第21天，通过内耳途径接受抗原的组也出现了显著但较小的增殖反应。相比之下，中耳途径未能产生细胞介导的免疫。这些研究表明，内耳是抗原加工的有效途径，可导致获得全身体液免疫和细胞免疫。反过来，全身免疫的发展已被发现对内耳具有保护作用。
