Natarajan K, Muthukkaruppan V R
Dev Comp Immunol. 1985 Spring;9(2):301-10. doi: 10.1016/0145-305x(85)90121-1.
Surface immunoglobulin bearing (sIg+) cells were identified in the lizard Calotes versicolor by immunofluorescence using a polyvalent antiserum to lizard immunoglobulins and class-specific antibodies to lizard IgM and IgY. 53.3 +/- 1.6% of spleen cells, 23.6 +/- 0.8% of peripheral blood mononuclear cells, 21.5 +/- 1.8% of bone marrow mononuclear cells and less than 1% of thymus cells were found to bear immunoglobulin (Ig) on their surface. A similar proportion of cells in each tissue was stained with rabbit anti-lizard mu (specific for IgM) whereas only a small proportion of cells were stained with rabbit anti-lizard (specific for IgY). Adult thymectomy significantly increased the proportion of sIgM+ cells in spleen and blood whereas high dose cyclophosphamide (300mg/kg body weight) decreased the proportion of sIgM+ cells thus suggesting that sIg+ cells in the lizard are of B cell lineage as in higher vertebrates. Ontogenetic studies indicate that embryonic liver is an organ enriched for sIgM+ cells at certain stages of development and thus suggest liver to be a site for differentiation of sIg+ cells in lizard embryos.
利用针对蜥蜴免疫球蛋白的多价抗血清以及针对蜥蜴IgM和IgY的类特异性抗体，通过免疫荧光法在变色树蜥中鉴定出表面带有免疫球蛋白（sIg+）的细胞。发现53.3±1.6%的脾细胞、23.6±0.8%的外周血单核细胞、21.5±1.8%的骨髓单核细胞以及不到1%的胸腺细胞表面带有免疫球蛋白（Ig）。每个组织中相似比例的细胞用兔抗蜥蜴μ链（对IgM特异）染色，而只有一小部分细胞用兔抗蜥蜴（对IgY特异）染色。成年蜥蜴胸腺切除显著增加了脾和血液中sIgM+细胞的比例，而高剂量环磷酰胺（300mg/kg体重）降低了sIgM+细胞的比例，因此表明蜥蜴中的sIg+细胞与高等脊椎动物一样属于B细胞谱系。个体发育研究表明，胚胎肝脏在发育的某些阶段是富含sIgM+细胞的器官，因此提示肝脏是蜥蜴胚胎中sIg+细胞的分化部位。
