Manickasundari M, Pitchappan R M
Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, India.
Dev Comp Immunol. 1987 Summer;11(3):605-12. doi: 10.1016/0145-305x(87)90049-8.
In an attempt to study the functions of thymus derived cell lineage, two different heterologous anti-lizard thymocyte sera (ATS i.v. & ATS i.p) were raised by immunizing rabbits with viable thymocytes of Calotes versicolor. Cytotoxicity assay, nylon wool fractionation and thymectomy were employed to assess the effect of these ATS on lymphoid cells of the lizards. The results revealed that ATS i.v. contained minimum amount of irrelevant antibodies than ATS i.p; non-adherent splenocytes were enriched with ATS sensitive cells, whereas the adherent with SIg+; adult thymectomy decreased ATS sensitive cells in spleens and the density of thymic antigen (identified by the ATS) in splenic thymus derived cell lineage was much higher than on thymocytes themselves. The cells of thymic lineage identified by the ATS thus resemble those of higher vertebrates.
为了研究胸腺来源细胞系的功能，用变色树蜥的活胸腺细胞免疫家兔，制备了两种不同的异源抗蜥蜴胸腺细胞血清（静脉注射抗血清和腹腔注射抗血清）。采用细胞毒性试验、尼龙毛分级分离法和胸腺切除术来评估这些抗血清对蜥蜴淋巴细胞的作用。结果显示，静脉注射抗血清比腹腔注射抗血清含有更少的无关抗体；非黏附性脾细胞富含对抗血清敏感的细胞，而黏附性脾细胞富含表面免疫球蛋白阳性细胞；成年蜥蜴胸腺切除术后，脾脏中对抗血清敏感的细胞减少，脾脏中胸腺来源细胞系的胸腺抗原（由抗血清鉴定）密度远高于胸腺细胞本身。因此，由抗血清鉴定的胸腺细胞系细胞类似于高等脊椎动物的细胞。
