Department of Biomedical Engineering, Michigan Technological University, Houghton, Michigan 49931, United States.
Department of Chemical Engineering, Michigan Technological University, Houghton, Michigan 49931, United States.
ACS Appl Mater Interfaces. 2024 May 29;16(21):26998-27010. doi: 10.1021/acsami.4c00213. Epub 2024 May 15.
A coating that can be activated by moisture found in respiratory droplets could be a convenient and effective way to control the spread of airborne pathogens and reduce fomite transmission. Here, the ability of a novel 6-hydroxycatechol-containing polymer to function as a self-disinfecting coating on the surface of polypropylene (PP) fabric was explored. Catechol is the main adhesive molecule found in mussel adhesive proteins. Molecular oxygen found in an aqueous solution can oxidize catechol and generate a known disinfectant, hydrogen peroxide (HO), as a byproduct. However, given the limited amount of moisture found in respiratory droplets, there is a need to enhance the rate of catechol autoxidation to generate antipathogenic levels of HO. 6-Hydroxycatechol contains an electron donating hydroxyl group on the 6-position of the benzene ring, which makes catechol more susceptible to autoxidation. 6-Hydroxycatechol-coated PP generated over 3000 μM of HO within 1 h when hydrated with a small amount of aqueous solution (100 μL of PBS). The generated HO was three orders of magnitude higher when compared to the amount generated by unmodified catechol. 6-Hydroxycatechol-containing coating demonstrated a more effective antimicrobial effect against both Gram-positive ( and ) and Gram-negative ( and ) bacteria when compared to unmodified catechol. Similarly, the self-disinfecting coating reduced the infectivity of both bovine viral diarrhea virus and human coronavirus 229E by as much as a 2.5 log reduction value (a 99.7% reduction in viral load). Coatings containing unmodified catechol did not generate sufficient HO to demonstrate significant virucidal effects. 6-Hydroxycatechol-containing coating can potentially function as a self-disinfecting coating that can be activated by the moisture present in respiratory droplets to generate HO for disinfecting a broad range of pathogens.
一种可以被呼吸道飞沫中的水分激活的涂层，可能是控制空气传播病原体传播和减少接触传播的一种便捷有效的方法。在这里，研究了一种新型的含有 6-羟基儿茶酚的聚合物在聚丙烯 (PP) 织物表面作为自消毒涂层的功能。儿茶酚是贻贝类黏附蛋白中主要的黏附分子。水溶液中的分子氧可以氧化儿茶酚并生成已知的消毒剂过氧化氢 (HO) 作为副产物。然而，考虑到呼吸道飞沫中水分的含量有限，需要提高儿茶酚自氧化的速度，以产生抗病原体水平的 HO。6-羟基儿茶酚在苯环的 6 位上含有一个供电子的羟基，这使得儿茶酚更容易发生自氧化。当用少量水溶液 (100 μL PBS) 水合时，6-羟基儿茶酚涂覆的 PP 在 1 小时内产生超过 3000 μM 的 HO。与未修饰的儿茶酚相比，生成的 HO 高出三个数量级。与未修饰的儿茶酚相比，含有 6-羟基儿茶酚的涂层对革兰氏阳性菌（和）和革兰氏阴性菌（和）都表现出更有效的抗菌效果。同样，自消毒涂层使牛病毒性腹泻病毒和人冠状病毒 229E 的感染力降低了 2.5 个对数减少值（病毒载量降低 99.7%）。未修饰的儿茶酚的涂层没有产生足够的 HO 来表现出显著的病毒杀灭效果。含有 6-羟基儿茶酚的涂层可能可以作为一种自消毒涂层，通过呼吸道飞沫中存在的水分激活，产生 HO 来消毒广泛的病原体。
