Giphart-Gassler M, Van de Putte P
Gene. 1979 Sep;7(1):33-50. doi: 10.1016/0378-1119(79)90041-6.
An EcoRI fragment, containing approx. 5100 base pairs (bp) of the immunity-end of bacteriophage Mu, was inserted into the multicopy plasmid pMB9 by in vitro recombination. The expression of early Mu genes, located on the cloned fragment, is thermo-inducible because of the presence of the ts mutation in gene c. The isolation of a transformant harbouring the recombinant plasmid, pGP1, was possible only when expression of Mu genes was prevented. pGP1 can be maintained at 28 degrees C at high copy number, but at 42 degrees C the pGP1 containing cells are killed due to the expression of the kil gene of Mu. The following Mu genes are present on pGP1: the ner gene, the integration and replication genes A and B, the cim gene, and the kil gene. pGP1 containing cells do not show Gam and Sot activity at 42 degrees C, therefore the leftmost EcoRI site on the Mu DNA is located between genes kil and gam or sot, or within the gam or sot gene.
一个含有噬菌体 Mu 免疫末端约 5100 个碱基对（bp）的 EcoRI 片段，通过体外重组插入到多拷贝质粒 pMB9 中。由于基因 c 中存在温度敏感突变，位于克隆片段上的早期 Mu 基因的表达是热诱导性的。只有当 Mu 基因的表达被阻止时，才有可能分离到携带重组质粒 pGP1 的转化体。pGP1 可以在 28℃下以高拷贝数维持，但在 42℃时，含有 pGP1 的细胞会因 Mu 的 kil 基因表达而死亡。pGP1 上存在以下 Mu 基因：ner 基因、整合和复制基因 A 和 B、cim 基因以及 kil 基因。含有 pGP1 的细胞在 42℃时不显示 Gam 和 Sot 活性，因此 Mu DNA 上最左边的 EcoRI 位点位于 kil 基因与 gam 或 sot 基因之间，或者在 gam 或 sot 基因内。
