Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
J Invest Dermatol. 2024 Jul;144(7):1430-1439. doi: 10.1016/j.jid.2024.04.003. Epub 2024 May 15.
Cutaneous wounds are common injuries that affect millions of people around the world. In vulnerable populations such as the elderly and those with diabetes, defects in wound healing can lead to the development of chronic open wounds. Although mammalian models are commonly used to study cutaneous wound healing, the challenges of in vivo imaging in mammals have hampered detailed observation of cell coordination and cell signaling during wound healing. The zebrafish is becoming increasingly popular for studying cutaneous wound healing owing to its genetic accessibility, suitability for experimental manipulation, and the ability to perform live, in vivo imaging with cellular or even subcellular resolution. In this paper, we review some of the techniques that have been developed for eliciting cutaneous wounds in the zebrafish, including an economical method we recently developed using a rotary tool that generates consistent and reproducible full-thickness wounds. Combined with the thousands of transgenic lines and experimental assays available in zebrafish, the ability to generate reproducible cutaneous wounds makes it possible to study key cellular and molecular events during wound healing using this powerful experimental model organism.
皮肤创伤是一种常见的伤害，影响着全世界数以百万计的人。在易受伤害的人群中，如老年人和糖尿病患者，伤口愈合的缺陷会导致慢性开放性伤口的发展。尽管哺乳动物模型常用于研究皮肤创伤愈合，但在哺乳动物中进行体内成像的挑战阻碍了对创伤愈合过程中细胞协调和细胞信号传递的详细观察。由于其遗传可及性、适合实验操作以及进行活体、体内成像的能力，甚至具有细胞或亚细胞分辨率，斑马鱼越来越受欢迎，可用于研究皮肤创伤愈合。在本文中，我们回顾了一些在斑马鱼中诱发皮肤创伤的技术，包括我们最近使用旋转工具开发的一种经济高效的方法，该方法可生成一致且可重复的全层伤口。结合斑马鱼中数千种转基因系和实验检测，生成可重复的皮肤创伤的能力使得使用这种强大的实验模式生物研究创伤愈合过程中的关键细胞和分子事件成为可能。
