Li Xin-Lian, Zhang Jing-Jing, Li Dou-Dou, Cai Xin-Yan, Qi Yi-Xiang, Lu Yong-Yue
Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2024 May 2;15:1362089. doi: 10.3389/fmicb.2024.1362089. eCollection 2024.
Entomopathogenic fungi (EPF) are economical and environmentally friendly, forming an essential part of integrated pest management strategies. We screened six strains of (B1-B6) (Hypocreales: Cordycipitaceae), of which B4 was the most virulent to (Hendel) (Diptera: Tephritidae). We further assessed the biological characteristics of strain B4 and the environmental factors influencing its ability to infect . We also evaluated the effects of B4 on two of the natural predators of . We found that strain B4 was the most virulent to 3rd instar larvae, pupae, and adult , causing mortality rates of 52.67, 61.33, and 90.67%, respectively. B4 was not toxic to eggs. The optimum B4 effects on were achieved at a relative humidity of 91-100% and a temperature of 25°C. Among the six insecticides commonly used for control, 1.8% abamectin emulsifiable concentrate had the strongest inhibitory effect on B4 strain germination. B4 spraying affected both natural enemies ( and ), reducing the number of and killing adults. We found a valuable strain of EPF (B4) that is virulent against many life stages of and has great potential for the biological control of . We also provide an important theoretical and practical base for developing a potential fungicide to control .
昆虫病原真菌（EPF）经济且环保，是害虫综合治理策略的重要组成部分。我们筛选了6株菌（B1 - B6）（肉座菌目：虫草科），其中B4对地中海实蝇（亨德尔）（双翅目：实蝇科）的毒性最强。我们进一步评估了B4菌株的生物学特性以及影响其感染地中海实蝇能力的环境因素。我们还评估了B4对地中海实蝇两种自然天敌的影响。我们发现B4菌株对3龄幼虫、蛹和成虫地中海实蝇的毒性最强，死亡率分别为52.67%、61.33%和90.67%。B4对地中海实蝇卵无毒。在相对湿度91 - 100%和温度25°C条件下，B4对地中海实蝇的防治效果最佳。在常用于防治地中海实蝇的6种杀虫剂中，1.8%阿维菌素乳油对B4菌株萌发的抑制作用最强。喷洒B4会影响两种天敌（[天敌名称1]和[天敌名称2]），减少[天敌名称1]数量并杀死[天敌名称2]成虫。我们发现了一种有价值的昆虫病原真菌菌株（B4），它对地中海实蝇的多个生命阶段都有毒性，在生物防治地中海实蝇方面具有巨大潜力。我们还为开发一种潜在的防治地中海实蝇的杀菌剂提供了重要的理论和实践基础。
