Hanazawa S, Ohmori Y, Amano S, Miyoshi T, Kumegawa M, Kitano S
Biochem Biophys Res Commun. 1985 Sep 16;131(2):774-9. doi: 10.1016/0006-291x(85)91306-3.
We have investigated the role of interleukin-1 in the regulatory mechanisms of a bone remodeling system. Osteoblastic cell line (MC3T3-E1) established from newborn mouse calvaria spontaneously produced interleukin-1-like cytokine. Although the interleukin-1-like activity was not observed in culture supernatants of the cells during their exponential phase of growth, a most remarkable interleukin-1-like activity was detected in the supernatants of cells cultured in serum-free alpha-MEM on day 5 after the cells had formed a confluent monolayer. Gel filtration analysis indicated that the interleukin-1-like cytokine exhibits some heterogeneity in size.
我们研究了白细胞介素-1在骨重塑系统调节机制中的作用。从小鼠新生颅骨建立的成骨细胞系（MC3T3-E1）可自发产生白细胞介素-1样细胞因子。尽管在细胞生长的指数期，其培养上清液中未观察到白细胞介素-1样活性，但在细胞形成汇合单层后第5天，在无血清α-MEM中培养的细胞上清液中检测到了非常显著的白细胞介素-1样活性。凝胶过滤分析表明，白细胞介素-1样细胞因子在大小上表现出一定的异质性。
