Miyatake S, Otsuka T, Yokota T, Lee F, Arai K
EMBO J. 1985 Oct;4(10):2561-8. doi: 10.1002/j.1460-2075.1985.tb03971.x.
A cDNA clone that expresses granulocyte-macrophage colony stimulating factor (GM-CSF) activity in COS-7 cells has been isolated from a pcD library prepared from mRNA derived from concanavalin A-activated mouse helper T cell clones. Based on homology with the mouse GM-CSF cDNA sequence, the mouse GM-CSF gene was isolated. The human GM-CSF gene was also isolated based on homology with the human GM-CSF cDNA sequence. The nucleotide sequences determined for the genes and their flanking regions revealed that both the mouse and human GM-CSF genes are composed of three introns and four exons. The organization of the mouse and human GM-CSF genes are highly homologous and strong sequence homology between the two genes is found both in the coding and non-coding regions. A 'TATA'-like sequence was found 20-25 bp upstream from the transcription initiation site. In the 5'-flanking region, there is a highly homologous region extending 330 bp upstream of the putative TATA box. This sequence may play a role in regulation of expression of the GM-CSF gene. These structures are compared with those of different lymphokine genes and their regulatory regions.
已从用伴刀豆球蛋白A激活的小鼠辅助性T细胞克隆的mRNA构建的pcD文库中分离出一个在COS - 7细胞中表达粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）活性的cDNA克隆。基于与小鼠GM - CSF cDNA序列的同源性，分离出了小鼠GM - CSF基因。还基于与人类GM - CSF cDNA序列的同源性分离出了人类GM - CSF基因。对这些基因及其侧翼区域测定的核苷酸序列表明，小鼠和人类GM - CSF基因均由三个内含子和四个外显子组成。小鼠和人类GM - CSF基因的结构高度同源，并且在编码区和非编码区都发现了两个基因之间很强的序列同源性。在转录起始位点上游20 - 25 bp处发现了一个类似“TATA”的序列。在5'侧翼区域，有一个高度同源区域，在假定的TATA框上游延伸330 bp。该序列可能在GM - CSF基因表达的调控中起作用。将这些结构与不同淋巴因子基因及其调控区域的结构进行了比较。
