Biesiadecki Marek, Mołoń Mateusz, Balawender Krzysztof, Kobylińska Zofia, Galiniak Sabina
Institute of Medical Sciences, Rzeszów University, Rzeszów, Poland.
Institute of Biology, Rzeszów University, Rzeszów, Poland.
Front Oncol. 2024 May 7;14:1393078. doi: 10.3389/fonc.2024.1393078. eCollection 2024.
Data on oxidative protein damage, total antioxidant capacity (TAC) and lipid peroxidation in progression of prostate cancer remain elusive. So far, the influence of the presence of perineural invasion on the level of oxidative stress has not been described. Additionally, there is limited data on the level of oxidative stress in patients' urine.
We compared the levels of oxidative stress markers in serum and urine in 50 patients with prostate cancer depending on the tumor stage and histological grade, the Gleason score, and the presence of perineural invasion.
We found a significantly de-creased level of serum thiol groups and TAC in participants with prostate cancer. Similarly, serum Amadori products and malondialdehyde (MDA) were higher in patients than in healthy men. There was a significantly decrease in TAC and a significantly increased MDA in the urine of prostate cancer patients. As the stage of cancer increased, a decrease in the thiol group concentration and TAC as well as an increase in the concentration of lipid peroxidation products in the serum was observed. The serum level of advanced oxidation protein products (AOPP) increased in the group with Gleason scores greater than 7. Furthermore, serum thiol groups and TAC were reduced in the group with Gleason >7 as compared to Gleason <7. The presence of perineural invasion significantly reduced serum and urinary TAC and increased urinary AOPP concentration.
These results indicate a significant role for oxidative damage in prostate carcinogenesis and its progression. Characterizing oxidative and nitrosative damage to proteins may be useful in designing targeted therapies for prostate cancer patients.
关于前列腺癌进展过程中氧化蛋白损伤、总抗氧化能力（TAC）和脂质过氧化的数据仍然缺乏。到目前为止，尚未描述神经周围浸润的存在对氧化应激水平的影响。此外，关于患者尿液中氧化应激水平的数据有限。
我们比较了50例前列腺癌患者血清和尿液中氧化应激标志物的水平，这些患者根据肿瘤分期、组织学分级、Gleason评分以及神经周围浸润的存在情况进行分组。
我们发现前列腺癌患者血清巯基和TAC水平显著降低。同样，患者血清中的阿马多里产物和丙二醛（MDA）高于健康男性。前列腺癌患者尿液中的TAC显著降低，MDA显著升高。随着癌症分期增加，观察到血清中巯基浓度和TAC降低，脂质过氧化产物浓度升高。Gleason评分大于7的组中晚期氧化蛋白产物（AOPP）的血清水平升高。此外，与Gleason评分小于7的组相比，Gleason评分大于7的组中血清巯基和TAC降低。神经周围浸润的存在显著降低了血清和尿液中的TAC，并增加了尿液中AOPP的浓度。
这些结果表明氧化损伤在前列腺癌发生及其进展中起重要作用。表征蛋白质的氧化和亚硝化损伤可能有助于为前列腺癌患者设计靶向治疗方案。
