Programa de Pós-Graduação em Odontologia, Universidade Federal de Pelotas (UFPel), Pelotas- RS- Brasil.
Programa de Pós-graduação em Odontologia, Universidade Estadual de Campinas (UNICAMP), Campinas- SP- Brasil.
Braz Dent J. 2024 May 10;35:e245550. doi: 10.1590/0103-6440202405550. eCollection 2024.
This in vitro study aimed to determine the efficacy of dentin bonding agents in preventing color changes following Regenerative Endodontic Procedures. One hundred twenty bovine incisors were endodontically prepared and randomly assigned to a two main factors design: application of a dentin bonding agent (Scotchbond Adper, 3M ESPE, St Paul, MN, USA) in the pulp chamber (Group 1, n=60) versus no bonding intervention (Group 2, n=60), and five levels of intracanal medication (n=12/subgroup): Triple antibiotic paste (TAP), double antibiotic paste (DAB), calcium hydroxide (CH), modified triple antibiotic paste (TAPM), and Control (CTL). Color changes were measured over 28 days at multiple time points (1, 3, 7, 14, 21, and 28 days) using the CIEDE2000 formula to calculate the color difference (ΔE00) from baseline (T0). The ΔE00 quantifies the perceptible color difference between the initial and final tooth color, with lower values indicating less discoloration. The results were analyzed using repeated measures ANOVA-2 and post-hoc Holm-Sidak tests. The TAP subgroups, both with and without the bonding agent, exhibited the highest color variation. However, a pulp chamber seal with a bonding agent showed a protective effect against discoloration compared to no seal, even though complete prevention was not achieved. All groups demonstrated ΔE00 values beyond acceptable interpretation thresholds for clinical application, primarily driven by a reduction in lightness (L*) and a decrease in redness (a* value, shifting towards green). In conclusion, while the pulp chamber seal with a bonding agent mitigated TAP-induced discoloration, it did not eliminate it.
本体外研究旨在确定牙本质粘结剂在预防再生牙髓治疗后颜色变化方面的效果。将 120 颗牛切牙进行牙髓预备，并随机分为两个主要因素设计：在牙髓室内应用牙本质粘结剂（Scotchbond Adper，3M ESPE，St Paul，MN，USA）（第 1 组，n=60）与不进行粘结干预（第 2 组，n=60），以及五种根管内药物水平（n=12/亚组）：三抗生素糊剂（TAP）、双抗生素糊剂（DAB）、氢氧化钙（CH）、改良三抗生素糊剂（TAPM）和对照（CTL）。使用 CIEDE2000 公式在多个时间点（1、3、7、14、21 和 28 天）测量颜色变化，从基线（T0）计算颜色差异（ΔE00）。ΔE00 量化了初始和最终牙齿颜色之间可感知的颜色差异，较低的值表示变色较少。结果使用重复测量方差分析-2 和事后 Holm-Sidak 检验进行分析。应用粘结剂的 TAP 亚组和未应用粘结剂的 TAP 亚组均表现出最高的颜色变化。然而，与无密封相比，用粘结剂密封牙髓腔对变色具有保护作用，尽管未能完全预防。所有组均显示出超出临床应用可接受解释阈值的ΔE00 值，主要由明度（L*）降低和红色度（a*值降低，向绿色偏移）驱动。总之，虽然用粘结剂密封牙髓腔减轻了 TAP 引起的变色，但并未完全消除。
