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获得性免疫缺陷综合征逆转录病毒p24 gag蛋白的细菌表达及其作为诊断试剂的用途。

Bacterial expression of the acquired immunodeficiency syndrome retrovirus p24 gag protein and its use as a diagnostic reagent.

作者信息

Dowbenko D J, Bell J R, Benton C V, Groopman J E, Nguyen H, Vetterlein D, Capon D J, Lasky L A

出版信息

Proc Natl Acad Sci U S A. 1985 Nov;82(22):7748-52. doi: 10.1073/pnas.82.22.7748.

DOI:10.1073/pnas.82.22.7748
PMID:3877932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC391411/
Abstract

A retrovirus [lymphoadenopathy-associated virus, human T-cell leukemia virus type III, acquired immunodeficiency syndrome (AIDS)-related virus] suspected of causing AIDS has been isolated recently. The detection of exposure to this retrovirus in donors of various blood products is important to prevent transmission of the disease from these donors to recipients. In the majority of cases, the detection of antibodies directed against either the viral core protein, a Mr approximately equal to 24,000 protein termed p24 gag, or the viral envelope antigen is proof of previous viral infection. Thus, we have expressed the p24 gag antigen in Escherichia coli in order to produce a diagnostic reagent for the detection of virus exposure. The bacterially synthesized antigen reacts with human and rabbit antisera directed against the native p24 gag protein in both electrophoretic transfer blot assay and ELISA. In addition, the use of bacterially produced antigens for ELISAs gave results that were comparable to those obtained by using antigens isolated from the virus.

摘要

一种怀疑导致艾滋病的逆转录病毒（淋巴结病相关病毒、人类T细胞白血病病毒III型、获得性免疫缺陷综合征（艾滋病）相关病毒）最近已被分离出来。检测各种血液制品捐献者是否接触过这种逆转录病毒对于防止疾病从这些捐献者传播给接受者很重要。在大多数情况下，检测针对病毒核心蛋白（一种分子量约为24000的称为p24 gag的蛋白）或病毒包膜抗原的抗体可证明先前曾感染过病毒。因此，我们在大肠杆菌中表达了p24 gag抗原，以便生产一种用于检测病毒接触情况的诊断试剂。细菌合成的抗原在电泳转移印迹分析和酶联免疫吸附测定中均能与人及兔抗天然p24 gag蛋白的抗血清发生反应。此外，将细菌产生的抗原用于酶联免疫吸附测定所得到的结果与使用从病毒中分离的抗原所得到的结果相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/2890f5f3be8f/pnas00362-0284-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/ff93df8d8fd4/pnas00362-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/f74d58f0929b/pnas00362-0284-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/2890f5f3be8f/pnas00362-0284-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/ff93df8d8fd4/pnas00362-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/f74d58f0929b/pnas00362-0284-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206d/391411/2890f5f3be8f/pnas00362-0284-c.jpg

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