Barreto Rodrigo Sn, Romagnolli Patricia, Fratini Paula, Mess Andrea Maria, Miglino Maria Angelica
School of Veterinary Medicine and Animal Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Federal University of the Southern Frontier, Realeza-PR, Brazil.
J Tissue Eng. 2019 Aug 8;10:2041731419867962. doi: 10.1177/2041731419867962. eCollection 2019 Jan-Dec.
The rich extracellular matrix (ECM) and availability make placenta eligible as alternative biomaterial source. Herein we produced placental mouse scaffolds by decellularization, and structure, composition, and cytocompatibility were evaluated to be considered as a biomaterial. We obtained a cell-free scaffold containing 9.42 ± 5.2 ng dsDNA per mg of ECM, presenting well-preserved structure and composition. Proteoglycans were widespread throughout ECM without cell nuclei and cell remnants. Collagen I, weak in native placenta, clearly appears in the scaffold after recellularization, opposite distribution was observed for collagen III. Fibronectin was well-observed in placental scaffolds whereas laminin and collagen IV were strong expressed. Placental scaffolds recellularization potential was confirmed after mouse embryonic fibroblasts 3D dynamic culture, resulting in massive scaffold repopulation with cell-cell interactions, cell-matrix adhesion, and maintenance of natural morphology. Our small size scaffolds provide a useful tool for tissue engineering to produce grafts and organ fragments, as well as for cellular biology purposes for tridimensional culture substrate.
丰富的细胞外基质（ECM）及其可用性使胎盘有资格成为替代生物材料来源。在此，我们通过去细胞化制备了胎盘小鼠支架，并对其结构、组成和细胞相容性进行了评估，以确定其是否可作为生物材料。我们获得了一种每毫克ECM含9.42±5.2纳克双链DNA的无细胞支架，其结构和组成保存良好。蛋白聚糖广泛分布于整个ECM中，无细胞核和细胞残余物。I型胶原蛋白在天然胎盘中含量较少，再细胞化后在支架中清晰可见，而III型胶原蛋白的分布则相反。在胎盘支架中可良好观察到纤连蛋白，而层粘连蛋白和IV型胶原蛋白表达较强。在小鼠胚胎成纤维细胞三维动态培养后，证实了胎盘支架的再细胞化潜力，导致大量细胞与支架重新结合，形成细胞间相互作用、细胞与基质粘附，并维持自然形态。我们的小尺寸支架为组织工程生产移植物和器官片段提供了有用工具，也为三维培养底物的细胞生物学研究提供了帮助。
