Taroncher Mercedes, Zingales Veronica, Rodríguez-Carrasco Yelko, Ruiz María José
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy and Food Science, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Research Group Alternative Methods for Determining Toxic Effects and Risk Assessment of Contaminants and Mixtures (RiskTox; GIUV2021-513), University of Valencia, 46100 València, Spain.
Foods. 2024 May 13;13(10):1501. doi: 10.3390/foods13101501.
The T-2 toxin (T-2) is a type A trichothecene found in cereals. The formation of metabolites is a frequent cause of mycotoxin-induced toxicity. In this work, the conversion of T-2 during biotransformation reactions in HepG2 cells was evaluated. For this, HepG2 cells were exposed to 30 (IC/2) and 60 (IC) nM of T-2 for 0, 1, 2, 3, 6, 8 and 24 h, and the concentrations of T-2 and its metabolites HT-2, T2-triol, T2-tetraol and neosolaniol were determined in both the cell fraction and culture medium through liquid chromatography coupled to high-resolution mass spectrometry-time of flight (LC-Q-TOF MS). Results showed a fast metabolization of T-2 (>90%) during the first 2 h, with HT-2 as its main (>95%) biotransformation product. The cell fraction showed higher levels ( < 0.05) of HT-2 (39.9 ± 2.1 nM) compared to the culture medium (12.53 ± 2.4 nM). This trend was also observed for the identified metabolites. T2-triol reached its maximum concentration (1.7 ± 0.4 nM) at 2 h, and at later times a time-dependent increase in the T2-tetraol and neosolaniol concentrations was observed. The identification of T-2 metabolites shows the need to continue combined toxicity studies of mycotoxins for a correct risk characterization of these natural contaminants.
T-2毒素（T-2）是一种在谷物中发现的A型单端孢霉烯族毒素。代谢产物的形成是霉菌毒素诱导毒性的常见原因。在这项研究中，评估了T-2在HepG2细胞生物转化反应中的转化情况。为此，将HepG2细胞暴露于30（IC/2）和60（IC）nM的T-2中0、1、2、3、6,、8和24小时，并通过液相色谱-高分辨率质谱-飞行时间联用（LC-Q-TOF MS）测定细胞组分和培养基中T-2及其代谢产物HT-2、T2-三醇、T2-四醇和新茄病镰刀菌烯醇的浓度。结果表明，在最初2小时内T-2快速代谢（>90%），HT-2是其主要（>95%）生物转化产物。与培养基（12.53±2.4 nM）相比，细胞组分中HT-2的水平更高（<0.05）（39.9±2.1 nM）。在所鉴定的代谢产物中也观察到了这种趋势。T2-三醇在2小时时达到最大浓度（1.7±0.4 nM），在随后的时间里，观察到T2-四醇和新茄病镰刀菌烯醇浓度随时间增加。T-2代谢产物的鉴定表明，需要继续开展霉菌毒素的联合毒性研究，以便对这些天然污染物进行正确的风险特征描述。
