Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, China.
Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Medicina (Kaunas). 2024 May 8;60(5):780. doi: 10.3390/medicina60050780.
: Connexin 43 (Cx43) is involved in the transfer of small signaling molecules between neighboring cells, thereby exerting a major influence on the initiation and progression of tumorigenesis. However, there is a lack of systematic research on Cx43 expression and its predictive role in clinical diagnosis and prognosis in pan-cancer. : Several biological databases were used to evaluate the expression levels of GJA1 (encoding Cx43) and its diagnostic and prognostic significance in pan-cancer. We targeted kidney renal clear cell carcinoma (KIRC) and investigated the relationship between GJA1 expression and different clinical features of KIRC patients. Then, we performed cell-based experiments to partially confirm our results and predicted several proteins that were functionally related to Cx43. : The expression of GJA1 has a high level of accuracy in predicting KIRC. High GJA1 expression was remarkably correlated with a favorable prognosis, and this expression was reduced in groups with poor clinical features in KIRC. Cell experiments confirmed the inhibitory effects of increased GJA1 expression on the migratory capacity of human renal cancer (RCC) cell lines, and protein-protein interaction (PPI) analysis predicted that CDH1 and CTNNB1 were closely related to Cx43. : GJA1 could be a promising independent favorable prognostic factor for KIRC, and upregulation of GJA1 expression could inhibit the migratory capacity of renal cancer cells.
间隙连接蛋白 43（Cx43）参与相邻细胞间小分子信号转导，从而对肿瘤发生的启动和进展产生重要影响。然而，在泛癌中，关于 Cx43 的表达及其在临床诊断和预后中的预测作用的系统性研究还很缺乏。
我们使用了几个生物数据库来评估 GJA1（编码 Cx43）的表达水平及其在泛癌中的诊断和预后意义。我们以肾透明细胞癌（KIRC）为研究对象，探讨了 GJA1 表达与 KIRC 患者不同临床特征之间的关系。然后，我们进行了基于细胞的实验，部分验证了我们的结果，并预测了几个与 Cx43 功能相关的蛋白质。
GJA1 的表达在预测 KIRC 方面具有较高的准确性。GJA1 高表达与预后良好显著相关，而在 KIRC 中具有不良临床特征的组中，这种表达水平降低。细胞实验证实了增加 GJA1 表达对人肾癌细胞系迁移能力的抑制作用，蛋白质-蛋白质相互作用（PPI）分析预测 CDH1 和 CTNNB1 与 Cx43 密切相关。
GJA1 可能是 KIRC 有前途的独立预后良好的因素，上调 GJA1 表达可抑制肾癌细胞的迁移能力。
