Cudziło Stanisław, Szermer-Olearnik Bożena, Dyjak Sławomir, Gratzke Mateusz, Sobczak Kamil, Wróblewska Anna, Szczygieł Agnieszka, Mierzejewska Jagoda, Węgierek-Ciura Katarzyna, Rapak Andrzej, Żeliszewska Paulina, Kozień Dawid, Pędzich Zbigniew, Pajtasz-Piasecka Elżbieta
Faculty of Advanced Technologies and Chemistry, Military University of Technology, 00-908 Warsaw, Poland.
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wrocław, Poland.
Materials (Basel). 2024 May 18;17(10):2438. doi: 10.3390/ma17102438.
In this research, we developed boron-rich nanoparticles that can be used for boron neutron capture therapy as potential carriers for boron delivery to cancerous tissues. Functionalized carbonated boron nitride nanostructures (CBNs) were successfully synthesized in self-propagating combustion waves in mixtures of high-nitrogen explosives and boron compounds. The products' composition, morphology, and structural features were investigated using Fourier transform infrared spectroscopy, powder X-ray diffraction, low-temperature nitrogen sorption analysis, thermogravimetric analysis, high-resolution scanning electron microscopy, and high-resolution transmission electron microscopy. The extreme conditions prevailing in combustion waves favor the formation of nanosized CBN hollow grains with highly disordered structures that are properly functionalized on the surface and inside the particles. Therefore, they are characterized by high porosity and good dispersibility in water, which are necessary for medical applications. During biological tests, a concentration-dependent effect of the obtained boron nitride preparations on the viability of normal and neoplastic cells was demonstrated. Moreover, the assessment of the degree of binding of fluorescently labeled nanoparticles to selected cells confirmed the relationships between the cell types and the concentration of the preparation at different incubation time points.
在本研究中，我们开发了富含硼的纳米颗粒，其可作为硼向癌组织递送的潜在载体用于硼中子俘获疗法。在高氮炸药与硼化合物的混合物中，通过自蔓延燃烧波成功合成了功能化的碳酸氮化硼纳米结构（CBNs）。使用傅里叶变换红外光谱、粉末X射线衍射、低温氮吸附分析、热重分析、高分辨率扫描电子显微镜和高分辨率透射电子显微镜对产物的组成、形态和结构特征进行了研究。燃烧波中存在的极端条件有利于形成具有高度无序结构的纳米级CBN空心颗粒，这些颗粒在表面和内部均得到了适当的功能化。因此，它们具有高孔隙率和在水中良好的分散性，这对于医学应用是必需的。在生物学测试中，证明了所得氮化硼制剂对正常细胞和肿瘤细胞活力具有浓度依赖性效应。此外，对荧光标记纳米颗粒与选定细胞的结合程度评估证实了在不同孵育时间点细胞类型与制剂浓度之间的关系。
