Kozień Dawid, Szermer-Olearnik Bożena, Rapak Andrzej, Szczygieł Agnieszka, Anger-Góra Natalia, Boratyński Janusz, Pajtasz-Piasecka Elżbieta, Bućko Mirosław M, Pędzich Zbigniew
Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30 Mickiewicz Av., 30-059 Kraków, Poland.
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Materials (Basel). 2021 Jun 2;14(11):3010. doi: 10.3390/ma14113010.
The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture therapy. Boron carbide powder was prepared by the direct reaction between boron and soot using the transport of reagents through the gas phase. The powder was ground, and a population of nanoparticles with an average particle size about 80 nm was selected by centrifugation. The aqueous suspension of the nanoparticles was functionalized with human immunoglobulins or FITC-labeled human immunoglobulins and was then added to the MC38 murine colon carcinoma and to the RAW 264.7 cell line of mouse macrophages. Flow cytometry analysis was used to determine interactions between the functionalized boron carbide nanoparticles and respective cells. It was shown that BC-IgG nanoconjugates may bind to phagocytic cells to be internalized by them, at least partially, whereas such nanoconjugates can only slightly interact with molecules on the cancer cells' surface.
这项工作的目的是研究富含硼的碳化硼纳米颗粒与选定的肿瘤细胞和免疫吞噬细胞之间的相互作用。进行实验以研究碳化硼纳米颗粒作为硼载体在硼中子俘获治疗中的应用可行性。通过硼与烟灰之间的直接反应，利用试剂在气相中的传输制备了碳化硼粉末。将该粉末研磨，通过离心选择平均粒径约为80nm的纳米颗粒群体。用人类免疫球蛋白或异硫氰酸荧光素标记的人类免疫球蛋白对纳米颗粒的水悬浮液进行功能化处理，然后将其添加到MC38小鼠结肠癌细胞和小鼠巨噬细胞RAW 264.7细胞系中。采用流式细胞术分析来确定功能化的碳化硼纳米颗粒与相应细胞之间的相互作用。结果表明，BC-IgG纳米缀合物可能与吞噬细胞结合，并至少部分地被它们内化，而这种纳米缀合物只能与癌细胞表面的分子发生轻微相互作用。
