Carlone N A, Borsotto M, Cuffini A M, Savoia D
G Batteriol Virol Immunol. 1985 Jul-Dec;78(7-12):185-204.
A number of bacterial strains (Escherichia coli, Proteus mirabilis and streptococci), obtained from patients with urinary infections, or from vaginal tampons, were assayed in order to evaluate their ability to adhere to uroepithelial cells. We employed, at a comparative purpose, two different methods to eliminate unattached bacteria: the centrifugation technique and the filtration technique. The effect of the pretreatment with subinhibitory concentrations of three chemotherapeutics widely employed in the urological practice (norfloxacin, pipemidic acid and co-trimoxazole) was determined on the strains which demonstrated a good rate of attachment. Growth in the presence of any of the three drugs decreased the adhesive properties of the microorganisms, reducing both the haemagglutination and the yeast aggregation titers of Gram-negative bacteria as well as their adhesiveness. Norfloxacin proved to be the most effective drug in affecting the pathogenicity characteristics of the assayed strains in comparison with the other two chemotherapeutics tested, even if they were also moderately able to inhibit the adhesiveness of the same strains.
为了评估从尿路感染患者或阴道棉塞中分离出的多种细菌菌株（大肠杆菌、奇异变形杆菌和链球菌）粘附于尿道上皮细胞的能力，我们进行了检测。为了进行比较，我们采用了两种不同的方法来去除未附着的细菌：离心技术和过滤技术。对于那些显示出良好附着率的菌株，我们测定了在泌尿外科实践中广泛使用的三种化疗药物（诺氟沙星、吡哌酸和复方新诺明）亚抑菌浓度预处理的效果。在这三种药物中的任何一种存在的情况下生长都会降低微生物的粘附特性，降低革兰氏阴性菌的血凝和酵母聚集效价以及它们的粘附性。与其他两种测试的化疗药物相比，诺氟沙星被证明是影响所检测菌株致病性特征最有效的药物，即使它们也能适度抑制相同菌株的粘附性。
