Obasi David C, Ogugua Victor N
Department of Biochemistry, Evangel University, Akaeze, Ebonyi State, Nigeria.
Department of Medical Biochemistry, David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria.
J Clin Exp Hepatol. 2024 May-Jun;14(3):100929. doi: 10.1016/j.jceh.2021.09.012. Epub 2021 Sep 23.
Ruzu herbal bitters (RHB) is a polyherbal mixture produced in Nigeria indicated for diabetes and other ailments. The consumers of the product testify of its efficacy, but there are not much scientific information on RHB. The study determined the effect of RHB on the liver function and lipid profile parameters of alloxan-induced diabetic rats.
Fifty-four adult albino rats were divided into nine groups of six rats each. Group 1 was the normal control, while groups 2-6 were diabetic. Group 2 was not treated, while groups 3-6 were respectively treated with 5 mg/kg b.w of glibenclamide, 0.14, 0.29, and 0.57 ml/kg b.w of RHB. Groups 7-9 were not diabetic but treated as groups 4-6. Diabetes was induced by intraperitoneal injection of freshly prepared alloxan into adult male albino Wister rats with a single dose of 120 mg/kg body weight. The blood sugar level, weight, liver function, and lipid profile of the rats were tested using standard methods.
The results showed a significant ( < 0.05) increase in the blood glucose level and decrease in weight in the diabetic-untreated group compared to the normal group. The liver function and lipid profile tests showed significant (<0.05) increases in the activities of gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST); increases in the levels of total bilirubin, total cholesterol (T.CHOL), triglycerides (TG), very low-density lipoprotein (VLDL) and lowdensity lipoprotein (LDL); decreases in the levels of total protein, albumin and high-density lipoproteins (HDL), in the diabetic-untreated group compared to the normal group. However, treatment of the diabetic rats with different doses of RHB caused the reversal of these effects to near-normal levels in a dose-dependent manner.
Our study reveals that RHB has antidiabetic, hepatoprotective, and antihyperlipidemic effects.
鲁祖草药苦味剂（RHB）是一种在尼日利亚生产的多草药混合物，用于治疗糖尿病和其他疾病。该产品的消费者证实了其疗效，但关于RHB的科学信息并不多。本研究确定了RHB对四氧嘧啶诱导的糖尿病大鼠肝功能和血脂参数的影响。
将54只成年白化大鼠分为9组，每组6只。第1组为正常对照组，第2 - 6组为糖尿病组。第2组未接受治疗，第3 - 6组分别用5mg/kg体重的格列本脲、0.14、0.29和0.57ml/kg体重的RHB进行治疗。第7 - 9组非糖尿病，但治疗方式同第4 - 6组。通过向成年雄性白化Wister大鼠腹腔注射单剂量120mg/kg体重的新鲜制备的四氧嘧啶诱导糖尿病。使用标准方法检测大鼠的血糖水平、体重、肝功能和血脂。
结果显示，与正常组相比，糖尿病未治疗组的血糖水平显著升高（<0.05），体重下降。肝功能和血脂检测显示，糖尿病未治疗组的γ-谷氨酰转移酶（GGT）、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性显著升高（<0.05）；总胆红素、总胆固醇（T.CHOL）、甘油三酯（TG）、极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）水平升高；总蛋白、白蛋白和高密度脂蛋白（HDL）水平降低。然而，用不同剂量的RHB治疗糖尿病大鼠可使这些影响以剂量依赖的方式逆转至接近正常水平。
我们的研究表明，RHB具有抗糖尿病、保肝和降血脂作用。
