Sarfraz Maliha, Khaliq Tanweer, Khan Junaid Ali, Aslam Bilal
Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Saudi Pharm J. 2017 May;25(4):449-452. doi: 10.1016/j.jsps.2017.04.004. Epub 2017 Apr 21.
The aim of the present study was to investigate the effects of aqueous extract of black pepper and ajwa seed on liver enzymes in alloxan-induced diabetic Wister albino rats to show the preventive and ameliorating effects in hyperglycemic rats. Rats were divided into 6 groups; normal control rats, diabetic control rats, glibenclamide treated rats, black pepper treated rats, ajwa seed treated rats and black pepper plus ajwa seed treated rats. Hyperglycemia was induced in the treatments groups by a single intraperitoneal injection of alloxan at 150 mg/kg body weight. The extracts were administered via oral incubation, doses were glibenclamide 10 mg/kg, black pepper 50 mg/kg, ajwa seed 500 mg/kg and their mixture 500 mg/kg body weight for a period of 8 weeks. Serum glucose, AST, ALT and ALP were assayed using spectrophotometric method. Results showed that ajwa seed and mixture significantly reduced glucose level. AST level was significantly reduced by mixture treated group. No significant difference was observed between different aqueous extract treated group in ALT and ALP level. The study indicates that black pepper and ajwa seed extract to some extend normalized the glucose and liver enzyme activities in alloxanized diabetic rats.
本研究的目的是探讨黑胡椒和阿月浑子种子水提取物对四氧嘧啶诱导的糖尿病Wister白化大鼠肝脏酶的影响，以显示其对高血糖大鼠的预防和改善作用。大鼠分为6组：正常对照大鼠、糖尿病对照大鼠、格列本脲治疗大鼠、黑胡椒治疗大鼠、阿月浑子种子治疗大鼠和黑胡椒加阿月浑子种子治疗大鼠。通过腹腔注射150mg/kg体重的四氧嘧啶诱导治疗组大鼠发生高血糖。提取物通过口服给药，剂量分别为格列本脲10mg/kg、黑胡椒50mg/kg、阿月浑子种子500mg/kg及其混合物500mg/kg体重，持续8周。采用分光光度法测定血清葡萄糖、AST、ALT和ALP。结果显示，阿月浑子种子及其混合物显著降低了血糖水平。混合物治疗组的AST水平显著降低。不同水提取物治疗组的ALT和ALP水平未观察到显著差异。该研究表明，黑胡椒和阿月浑子种子提取物在一定程度上使四氧嘧啶诱导的糖尿病大鼠的血糖和肝脏酶活性恢复正常。
