Department of Orthopedics, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.
Department of Nutrition, Affiliated Hospital of Jiangnan University, Wuxi, China.
Front Cell Infect Microbiol. 2024 May 13;14:1401963. doi: 10.3389/fcimb.2024.1401963. eCollection 2024.
The understanding of the link between the gut-bone axis is growing yearly, but the mechanisms involved are not yet clear. Our study analyzed the role of Sestrin2 (SESN2)pathway in the gut-bone axis. We established an osteoarthritis (OA) model in Sprague-Dawley (SD) rats using the anterior cruciate ligament transection (ACLT) procedure, followed by a dietary intervention with varying levels of dietary fiber content for 8 weeks. By 16S rRNA sequencing of the gut microbiota, we found that high dietary fiber (HDF) intake could significantly increase the Bacillota-dominant gut microbiota. Meanwhile, enzyme linked immunosorbent assay (ELISA) and histological analysis showed that intervention with HDF could reduce the degree of bone and joint lesions and inflammation. We hypothesize that HDF increased the dominant flora of Bacillota, up-regulated the expression of SESN2 in knee joint, and reduced gut permeability, thereby reducing systemic inflammatory response and the degree of bone and joint lesions. Therefore, the present study confirms that changes in gut microbiota induced by increased dietary fiber intake delayed the onset of OA by promoting up-regulation of SESN2 expression at the knee joint to maintain chondrocyte activity and reduce synovial inflammation.
对肠道-骨骼轴之间联系的理解逐年深入，但相关机制尚不清楚。我们的研究分析了 Sestrin2（SESN2）通路在肠道-骨骼轴中的作用。我们使用前交叉韧带切断（ACLT）手术在 Sprague-Dawley（SD）大鼠中建立骨关节炎（OA）模型，随后进行 8 周不同膳食纤维含量的饮食干预。通过对肠道微生物群的 16S rRNA 测序，我们发现高膳食纤维（HDF）摄入可显著增加以芽孢杆菌为主的肠道微生物群。同时，酶联免疫吸附测定（ELISA）和组织学分析表明，HDF 的干预可减轻骨和关节损伤及炎症的程度。我们假设 HDF 增加了芽孢杆菌的优势菌群，上调了膝关节 SESN2 的表达，降低了肠道通透性，从而减轻了全身炎症反应和骨与关节损伤的程度。因此，本研究证实，增加膳食纤维摄入引起的肠道微生物群变化通过促进膝关节 SESN2 表达的上调来延缓 OA 的发病，从而维持软骨细胞的活性并减轻滑膜炎症。
