鉴定由pKM101编码的、指定潜在致死基因产物的基因座。
Identification of pKM101-encoded loci specifying potentially lethal gene products.
作者信息
Winans S C, Walker G C
出版信息
J Bacteriol. 1985 Jan;161(1):417-24. doi: 10.1128/jb.161.1.417-424.1985.
Abstract
Two pKM101-encoded loci (designated kilA and kilB) have been identified which elaborate products that are potentially lethal to the bacterial cell. The lethal effects of each of these products is inhibited by two other plasmid-encoded loci, designated korA and korB (for kil override). Both korA and korB are required to control the lethality of either kil gene. In the presence of korA and korB both kil genes have other phenotypes: kilB is necessary for conjugal transfer, whereas kilA is responsible for the small-colony morphology on defined media that is characteristic of pKM101-containing strains (the Slo phenotype).
摘要
已鉴定出两个由pKM101编码的基因座（命名为kilA和kilB），它们所表达的产物可能对细菌细胞具有致死性。这两种产物的致死效应受到另外两个质粒编码的基因座（命名为korA和korB，意为kil抑制）的抑制。抑制kil基因的致死性需要korA和korB同时存在。在korA和korB存在的情况下，两个kil基因还有其他表型：kilB是接合转移所必需的，而kilA则导致在限定培养基上出现小菌落形态，这是含pKM101菌株的特征（Slo表型）。
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