变铅青链霉菌中的质粒转移：与pIJ101转移区域相关的一个致死-抑制系统的鉴定
Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pIJ101.
作者信息
Kendall K J, Cohen S N
出版信息
J Bacteriol. 1987 Sep;169(9):4177-83. doi: 10.1128/jb.169.9.4177-4183.1987.
Abstract
The 8.9-kilobase Streptomyces plasmid pIJ101 is self-transmissible at high frequency into recipient strains. By genetic analysis of the transfer region of the plasmid, we identified six plasmid-encoded loci involved in gene transfer and the associated pocking phenomenon. Two loci, kilA and kilB, could not be cloned into Streptomyces lividans on a minimal pIJ101-based replicon unless suitable kil-override (kor) genes were present, either in cis or in trans. korA could control the lethal effects of both kilA and kilB, whereas korB could control only the effects of kilB. KilB mutants were defective in their pocking reaction; kilA mutants produced no visible pocks whatsoever. Mutations in two other loci, tra and spd, produced no pocks and defective pocks, respectively. These results suggest that kilA, kilB, tra, and spd are intimately involved in plasmid transfer and that the actions of kilA and kilB are regulated by the products of the korA and korB genes.
摘要
8.9千碱基的链霉菌质粒pIJ101能够以高频自我转移到受体菌株中。通过对该质粒转移区域的遗传分析，我们鉴定出了六个参与基因转移及相关噬菌斑现象的质粒编码位点。除非存在合适的 kil- 抑制（kor）基因（顺式或反式），否则两个位点kilA和kilB无法基于最小的pIJ101复制子克隆到变铅青链霉菌中。korA能够控制kilA和kilB的致死效应，而korB只能控制kilB的效应。KilB突变体在噬菌斑反应中存在缺陷；kilA突变体则完全不产生可见的噬菌斑。另外两个位点tra和spd的突变分别产生无噬菌斑和有缺陷的噬菌斑。这些结果表明，kilA、kilB、tra和spd与质粒转移密切相关，并且kilA和kilB的作用受korA和korB基因产物的调控。
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