Karachewski N O, Busch E L, Wells C L
J Clin Microbiol. 1985 Jan;21(1):122-6. doi: 10.1128/jcm.21.1.122-126.1985.
This study evaluated the PRAS II, RapID ANA, and API 20A systems for the identification of anaerobic bacteria. A total of 80 isolates (68 fresh clinical isolates and 12 stock cultures) were examined and included 25 Bacteriodes spp., 7 Fusobacterium spp., 12 Clostridium spp., 2 Veillonella spp., 16 gram-positive cocci, and 18 gram-positive nonsporeforming bacilli. All isolates were initially identified by the procedures outlined in Holdeman et al. (ed.), Anaerobe Laboratory Manual, Virginia Polytechnic Institute and State University, Blacksburg, Va., 1977; identifications from the PRAS II, RapID ANA, and API 20A systems were compared with these initial identifications. If no supplemental tests were required, the RapID ANA and API 20A systems had incubation times of 4 and 24 h, respectively; the PRAS II system generally required 2 to 5 days of incubation, depending on the growth rate of the isolate. PRAS II identified 74% correct to species level, 14% correct to genus only, and 6% incorrect; 6% could not be identified. PRAS II data were reevaluated according to a revised data base that was provided after completion of the study; PRAS II (revised) identified 82% correct to species, 12% correct to genus only, and 6% incorrect. RapID ANA identified 62% correct to the species level, 28% correct to genus only, and 10% incorrect. API 20A identified 71% correct to the species level, 10% correct to genus only, and 3% incorrect; 16% could not identified. The API 20A is a more established system for identification of anaerobic bacteria; PRAS II and RapID ANA appear to be promising new methods for the identification of anaerobic bacteria.
本研究评估了PRAS II、RapID ANA和API 20A系统用于鉴定厌氧菌的性能。共检测了80株分离菌（68株新鲜临床分离株和12株保藏培养物），包括25株拟杆菌属、7株梭杆菌属、12株梭菌属、2株韦荣球菌属、16株革兰氏阳性球菌和18株革兰氏阳性无芽孢杆菌。所有分离菌最初均按照Holdeman等人（编著）的《厌氧菌实验室手册》（弗吉尼亚理工学院和州立大学，弗吉尼亚州布莱克斯堡，1977年）中所述程序进行鉴定；将PRAS II、RapID ANA和API 20A系统的鉴定结果与这些初始鉴定结果进行比较。如果无需进行补充试验，RapID ANA和API 20A系统的孵育时间分别为4小时和24小时；PRAS II系统通常需要2至5天的孵育时间，具体取决于分离菌的生长速度。PRAS II在种水平鉴定正确的比例为74%，仅在属水平鉴定正确的比例为14%，鉴定错误的比例为6%；6%无法鉴定。根据研究完成后提供的修订数据库对PRAS II数据进行了重新评估；PRAS II（修订版）在种水平鉴定正确的比例为82%，仅在属水平鉴定正确的比例为12%，鉴定错误的比例为6%。RapID ANA在种水平鉴定正确的比例为62%，仅在属水平鉴定正确的比例为28%，鉴定错误的比例为10%。API 20A在种水平鉴定正确的比例为71%，仅在属水平鉴定正确的比例为10%，鉴定错误的比例为3%；16%无法鉴定。API 20A是一种更为成熟的厌氧菌鉴定系统；PRAS II和RapID ANA似乎是有前景的厌氧菌鉴定新方法。
