Deng Zeshuai, Li Yuan, Gao Zhiwei, Zhang Zhiqiang, Yang Daode
Institute of Wildlife Conservation, Central South University of Forestry and Technology, Changsha, China.
Front Genet. 2024 May 20;15:1374263. doi: 10.3389/fgene.2024.1374263. eCollection 2024.
: an endemic amphibian of China, is found in the hills and mountains south of the Yangtze River. In this comprehensive study, we collected 162 samples from 14 different localities to delve into the genetic diversity of using mitochondrial Cytb and nuclear RAG2 as genetic markers. Our findings reveal that the Nanling Mountains, specifically regions like Jiuyi Shan, Jinggang Shan, Mang Shan, and Qiyun Shan, are genetic hotspots harboring remarkable diversity. The research results also indicate that there is gene flow among the various populations of the species, and no distinct population structure has formed, which may be due to migration. Moreover, populations in some regions, as well as the overall population, show signs of a possible genetic bottleneck, which we speculate may have been caused by climate change. However, given the exploratory nature of our study, further investigations are warranted to confirm these observations. Through phylogenetic analyses, we uncovered indications that might have originated within the Nanling region, dispersing along the east-west mountain ranges, with a significant contribution originating from Jiuyi Shan. The genetic distributions uncovered through our research reflect historical migratory patterns, evident in the distinct haplotypes of the RAG2 gene between the western and eastern parts of the studied area. Moreover, Heng Shan and Yangming Shan exhibited unique genetic signatures, possibly influenced by geographic isolation, which has shaped their distinct genotypes. The insights gained from this study hold profound implications for conservation efforts. By identifying regions rich in genetic diversity and crucial gene flow corridors, we can develop more effective conservation strategies. Preserving these genetically diverse areas, especially within the Nanling Mountains, is vital for maintaining the evolutionary potential of . In conclusion, our research has laid a solid foundation for understanding the genetic landscape of . , shedding light on its origins, population structures, and evolutionary trajectories. This knowledge will undoubtedly guide future research endeavors and inform conservation strategies for this endemic amphibian.
[物种名称]是中国的一种本土两栖动物，分布于长江以南的山区。在这项综合研究中，我们从14个不同地点收集了162个样本，以线粒体细胞色素b（Cytb）和核重组激活基因2（RAG2）作为遗传标记，深入研究[物种名称]的遗传多样性。我们的研究结果表明，南岭地区，特别是九嶷山、井冈山、莽山和齐云山等地，是具有显著多样性的遗传热点。研究结果还表明，该物种的各个种群之间存在基因流动，尚未形成明显的种群结构，这可能是由于迁移所致。此外，一些地区的种群以及整个种群都显示出可能存在遗传瓶颈的迹象，我们推测这可能是由气候变化引起的。然而，鉴于我们研究的探索性质，需要进一步调查以证实这些观察结果。通过系统发育分析，我们发现[物种名称]可能起源于南岭地区，沿着东西山脉扩散，其中九嶷山的贡献显著。我们研究中发现的遗传分布反映了历史迁徙模式，在所研究区域的西部和东部，RAG2基因的单倍型明显不同。此外，衡山和阳明山表现出独特的遗传特征，可能受到地理隔离的影响，从而形成了它们独特的基因型。这项研究获得的见解对保护工作具有深远意义。通过识别遗传多样性丰富的地区和关键的基因流动走廊，我们可以制定更有效的保护策略。保护这些遗传多样的地区，特别是南岭山脉内的地区，对于维持[物种名称]的进化潜力至关重要。总之，我们的研究为了解[物种名称]的遗传景观奠定了坚实的基础，揭示了其起源、种群结构和进化轨迹。这些知识无疑将指导未来的研究工作，并为这种本土两栖动物的保护策略提供参考。
