Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing 100102, China; Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing 100102, China.
Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing 100102, China; Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing 100102, China.
Int J Biol Macromol. 2024 Aug;275(Pt 1):132885. doi: 10.1016/j.ijbiomac.2024.132885. Epub 2024 Jun 3.
Fructose 1,6-bisphosphate aldolase (FBA) is a pivotal enzyme, which plays a critical role in fixing CO through the process of in the Calvin cycle. In this study, a comprehensive exploration of the FBA family genes in moso bamboo (Phyllostachys edulis) was conducted by the bioinformatics and biological analyses. A total of nine FBA genes (PeFBA1-PeFBA9) were identified in the moso bamboo genome. The expression patterns of PeFBAs across diverse tissues of moso bamboo suggested that they have multifaceted functionality. Notably, PeFBA8 might play an important role in regulating photosynthetic carbon metabolism. Co-expression and cis-element analyses demonstrated that PeFBA8 was regulated by a photosynthetic regulatory transcription factor (PeGLK1), which was confirmed by yeast one-hybrid and dual-luciferase assays. In-planta gene editing analysis revealed that the edited PeFBA8 mutants displayed compromised photosynthetic functionality, characterized by reduced electron transport rate and impaired photosystem I, leading to decreased photosynthesis rate overall, compared to the unedited control. The recombinant protein of PeFBA8 from prokaryotic expression exhibited enzymatic catalytic function. The findings suggest that the expression of PeFBA8 can affect photosynthetic efficiency of moso bamboo leaves, which underlines the potential of leveraging PeFBA8's regulatory mechanism to breed bamboo varieties with enhanced carbon fixation capability.
果糖-1,6-二磷酸醛缩酶（FBA）是一种关键酶，在卡尔文循环中通过固定 CO2 发挥着至关重要的作用。本研究通过生物信息学和生物学分析，对毛竹（Phyllostachys edulis）的 FBA 家族基因进行了全面探索。在毛竹基因组中鉴定出了 9 个 FBA 基因（PeFBA1-PeFBA9）。PeFBAs 在毛竹不同组织中的表达模式表明它们具有多方面的功能。特别是，PeFBA8 可能在调节光合作用碳代谢中发挥重要作用。共表达和顺式作用元件分析表明，PeFBA8 受到光合调节转录因子（PeGLK1）的调控，酵母单杂交和双荧光素酶测定实验对此进行了验证。在植物体内的基因编辑分析表明，编辑后的 PeFBA8 突变体表现出光合作用功能受损，其电子传递速率降低，光系统 I 受损，导致整体光合作用速率下降，与未编辑的对照相比。来自原核表达的 PeFBA8 重组蛋白表现出酶催化功能。研究结果表明，PeFBA8 的表达可以影响毛竹叶片的光合作用效率，这突显了利用 PeFBA8 的调控机制来培育具有增强碳固定能力的竹种的潜力。
