• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于正向遗传筛选以鉴定秀丽隐杆线虫生物学过程中涉及的新因子的方案。

Protocol for forward genetic screening to identify novel factors involved in a biological process in Caenorhabditis elegans.

机构信息

Department of Physiology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

Department of Physiology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

出版信息

STAR Protoc. 2024 Jun 21;5(2):103114. doi: 10.1016/j.xpro.2024.103114. Epub 2024 Jun 5.

DOI:10.1016/j.xpro.2024.103114
PMID:38843399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11216056/
Abstract

Forward genetic screens have been a powerful tool for discovering genes involved in various biological processes in Caenorhabditis elegans. Here, we present a protocol for forward genetic screening to identify novel factors involved in a biological process in C. elegans. We describe steps for mutagenesis, screening, and backcrossing. To save time and effort, we also detail procedures for utilizing whole-genome sequencing to exclude mutants of previously characterized genes from crosses for mapping mutations. For complete details on the use and execution of this protocol, please refer to Yoshida et al..

摘要

正向遗传学筛选一直是发现秀丽隐杆线虫各种生物学过程中相关基因的有力工具。在这里，我们提供了一个正向遗传学筛选的方案，用于鉴定秀丽隐杆线虫生物学过程中的新的因子。我们描述了诱变、筛选和回交的步骤。为了节省时间和精力，我们还详细介绍了利用全基因组测序排除已鉴定基因的突变体从杂交中进行突变定位的程序。有关该方案的使用和执行的完整详细信息，请参考 Yoshida 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/0593466e1ff4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/625cf6c39ef3/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/2587cbcb49c9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/9ecf49af4b65/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/7c64d615bf7b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/1d05da0abcb9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/f025f998a458/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/0593466e1ff4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/625cf6c39ef3/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/2587cbcb49c9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/9ecf49af4b65/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/7c64d615bf7b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/1d05da0abcb9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/f025f998a458/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d9/11216056/0593466e1ff4/gr6.jpg

相似文献

1
Protocol for forward genetic screening to identify novel factors involved in a biological process in Caenorhabditis elegans.用于正向遗传筛选以鉴定秀丽隐杆线虫生物学过程中涉及的新因子的方案。
STAR Protoc. 2024 Jun 21;5(2):103114. doi: 10.1016/j.xpro.2024.103114. Epub 2024 Jun 5.
2
Harnessing the power of genetics: fast forward genetics in Caenorhabditis elegans.利用遗传学的力量：秀丽隐杆线虫中的快速正向遗传学。
Mol Genet Genomics. 2021 Jan;296(1):1-20. doi: 10.1007/s00438-020-01721-6. Epub 2020 Sep 4.
3
Use of a Sibling Subtraction Method for Identifying Causal Mutations in by Whole-Genome Sequencing.使用同胞减法法通过全基因组测序鉴定因果突变。
G3 (Bethesda). 2018 Feb 2;8(2):669-678. doi: 10.1534/g3.117.300135.
4
MIP-MAP: High-Throughput Mapping of Temperature-Sensitive Mutants via Molecular Inversion Probes.MIP-MAP：通过分子倒置探针进行温度敏感突变体的高通量定位
Genetics. 2017 Oct;207(2):447-463. doi: 10.1534/genetics.117.300179. Epub 2017 Aug 21.
5
Whole genome sequencing and the transformation of C. elegans forward genetics.全基因组测序与秀丽隐杆线虫正向遗传学的变革。
Methods. 2014 Aug 1;68(3):437-40. doi: 10.1016/j.ymeth.2014.05.008. Epub 2014 May 27.
6
A machine learning enhanced EMS mutagenesis probability map for efficient identification of causal mutations in Caenorhabditis elegans.机器学习增强型 EMS 诱变概率图谱，可高效鉴定秀丽隐杆线虫中的因果突变。
PLoS Genet. 2024 Aug 26;20(8):e1011377. doi: 10.1371/journal.pgen.1011377. eCollection 2024 Aug.
7
Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans.用于鉴定秀丽隐杆线虫中EMS诱导突变的新一代测序技术
Curr Protoc Mol Biol. 2017 Jan 5;117:7.29.1-7.29.12. doi: 10.1002/cpmb.27.
8
Mutation Mapping and Identification by Whole-Genome Sequencing.全基因组测序的突变图谱与鉴定。
Methods Mol Biol. 2022;2468:257-269. doi: 10.1007/978-1-0716-2181-3_13.
9
TILLING is an effective reverse genetics technique for Caenorhabditis elegans.定向诱导基因组局部突变（TILLING）是一种用于秀丽隐杆线虫的有效反向遗传学技术。
BMC Genomics. 2006 Oct 18;7:262. doi: 10.1186/1471-2164-7-262.
10
Forward genetic screen of the C. elegans million mutation library reveals essential, cell-autonomous contributions of BBSome proteins to dopamine signaling.秀丽隐杆线虫百万突变文库的正向遗传学筛选揭示了 BBSome 蛋白对多巴胺信号传递的必需的、细胞自主的贡献。
J Neurochem. 2024 Sep;168(9):2073-2091. doi: 10.1111/jnc.16188. Epub 2024 Aug 8.

本文引用的文献

1
Distinct pathways for export of silencing RNA in systemic RNAi.系统性RNA干扰中沉默RNA的不同输出途径。
iScience. 2023 Sep 28;26(10):108067. doi: 10.1016/j.isci.2023.108067. eCollection 2023 Oct 20.
2
Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals.通过对 DNA 损伤反应缺陷动物进行诱变，高效地收集大量突变。
Sci Rep. 2021 Apr 7;11(1):7630. doi: 10.1038/s41598-021-87226-7.
3
A universal SNP and small-indel variant caller using deep neural networks.使用深度神经网络的通用 SNP 和小插入缺失变体调用器。
Nat Biotechnol. 2018 Nov;36(10):983-987. doi: 10.1038/nbt.4235. Epub 2018 Sep 24.
4
CloudMap: a cloud-based pipeline for analysis of mutant genome sequences.CloudMap：一种基于云的突变基因组序列分析流水线。
Genetics. 2012 Dec;192(4):1249-69. doi: 10.1534/genetics.112.144204. Epub 2012 Oct 10.
5
A strategy for direct mapping and identification of mutations by whole-genome sequencing.通过全基因组测序进行直接映射和突变鉴定的策略。
Genetics. 2010 Sep;186(1):427-30. doi: 10.1534/genetics.110.119230. Epub 2010 Jul 6.
6
Predicting deleterious amino acid substitutions.预测有害的氨基酸替换。
Genome Res. 2001 May;11(5):863-74. doi: 10.1101/gr.176601.
7
Mutagenesis.诱变
Methods Cell Biol. 1995;48:31-58.
8
The genetics of Caenorhabditis elegans.秀丽隐杆线虫的遗传学
Genetics. 1974 May;77(1):71-94. doi: 10.1093/genetics/77.1.71.