Abasht Behnam, Mutryn Marie F, Michalek Ryan D, Lee William R
Department of Animal and Food Sciences, University of Delaware, Newark, DE, United States of America.
Metabolon Inc., Durham, NC, United States of America.
PLoS One. 2016 Apr 20;11(4):e0153750. doi: 10.1371/journal.pone.0153750. eCollection 2016.
This study was conducted to characterize metabolic features of the breast muscle (pectoralis major) in chickens affected with the Wooden Breast myopathy. Live birds from two purebred chicken lines and one crossbred commercial broiler population were clinically examined by manual palpation of the breast muscle (pectoralis major) at 47-48 days of age. Metabolite abundance was determined by gas chromatography/mass spectrometry (GC/MS) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using breast muscle tissue samples from 16 affected and 16 unaffected chickens. Muscle glycogen content was also quantified in breast muscle tissue samples from affected and unaffected chickens. In total, levels of 140 biochemicals were significantly different (FDR<0.1 and fold-change A/U>1.3 or <0.77) between affected and unaffected chickens. Glycogen content measurements were considerably lower (1.7-fold) in samples taken from Wooden Breast affected birds when compared with samples from unaffected birds. Affected tissues exhibited biomarkers related to increased oxidative stress, elevated protein levels, muscle degradation, and altered glucose utilization. Affected muscle also showed elevated levels of hypoxanthine, xanthine, and urate molecules, the generation of which can contribute to altered redox homeostasis. In conclusion, our findings show that Wooden Breast affected tissues possess a unique metabolic signature. This unique profile may identify candidate biomarkers for diagnostic utilization and provide mechanistic insight into altered biochemical processes contributing to tissue hardening associated with the Wooden Breast myopathy in commercial chickens.
本研究旨在表征患有木胸肌病的鸡的胸肌（胸大肌）的代谢特征。在47-48日龄时，通过手动触诊胸肌（胸大肌）对来自两个纯种鸡系和一个杂交商品肉鸡群体的活禽进行临床检查。使用来自16只患病和16只未患病鸡的胸肌组织样本，通过气相色谱/质谱（GC/MS）和液相色谱-串联质谱（LC-MS/MS）测定代谢物丰度。还对患病和未患病鸡的胸肌组织样本中的肌肉糖原含量进行了定量。总体而言，患病鸡和未患病鸡之间140种生化物质的水平存在显著差异（FDR<0.1且变化倍数A/U>1.3或<0.77）。与未患病鸡的样本相比，从患有木胸病的鸡身上采集的样本中的糖原含量测量值显著更低（1.7倍）。患病组织表现出与氧化应激增加、蛋白质水平升高、肌肉降解和葡萄糖利用改变相关的生物标志物。患病肌肉中次黄嘌呤﹑黄嘌呤和尿酸分子的水平也升高，这些分子的产生可能导致氧化还原稳态的改变。总之，我们的研究结果表明，患有木胸病的组织具有独特的代谢特征。这种独特的特征可能有助于识别用于诊断的候选生物标志物，并为导致商品鸡木胸肌病相关组织硬化的生化过程改变提供机制性见解。
